miR-30d-5p调控人口腔鳞癌细胞增殖和凋亡的作用机制研究  

Role of MiR-30d-5p on the Proliferation and Apoptosis of TSCC1 cells

作　　者：周沛玲 严海超 游巧玲 延丽雅 周腾田 ZHOU Pei-ling;YAN Hai-chao;YOU Qiao-ling(Department of Stomatology,Dongguan Dalang Hospital,Dongguan,Guangdong,523770,China)

机构地区：[1]东莞市大朗医院口腔科,广东东莞523770

出　　处：《黑龙江医学》2020年第10期1350-1353,共4页Heilongjiang Medical Journal

基　　金：东莞市社会科技发展一般项目(东科201950715040913)。

摘　　要：目的探讨miR-30d-5p在调控人口腔鳞癌细胞(TSCC1)增殖和凋亡中发挥的作用。方法数据库分析与Beclin1相关的microRNA,qPCR验证miR-30d-5p和Beclin1在口腔鳞癌组织和对应癌旁组织中表达,并做相关性分析。培养TSCC1,转染miR-30d-5p mimic或miR-30d-5p inhibitor,MTT检测miR-30d-5p对TSCC1细胞增殖的调控,流式细胞计数检测细胞凋亡率。结果数据库分析显示miR-30d-5p和Beclin1呈强关联,qPCR验证miR-30d-5p在口腔鳞癌组织中高表达、Beclin1在口腔鳞癌组织中低表达,两者呈负相关(P<0.05)。转染miR-30d-5p能够促进TSCC1细胞增殖、抑制TSCC1细胞凋亡;而miR-30d-5p inhibitors抑制TSCC1细胞增殖、促进TSCC1细胞凋亡(P<0.05)。采用Lenti-sh-Beclin1的慢病毒转染TSCC1细胞后,TSCC1细胞增殖下降、凋亡升高(P<0.05)。结论miR-30d-5p可通过Beclin1调控TSCC1增殖和凋亡。Objective To investigate the role of miR-30d-5p on the proliferation and apoptosis of oral squamous cancer cells(TSCC1).Methods The microRNA related with Beclin1 was analyzed by database.Then qPCR was used to detect the expression of Beclin1 and miR-30d-5p in patients with oral squamous cell carcinoma.The association among Beclin1 and miR-30d-5p were analyzed with Chi-square test.TSCC1 cells were cultured.MiR-30d-5p mimic or miR-30d-5p inhibitor was transfected.MTT and flow cytometry were used to detect the proliferation and apoptosis of TSCC1 cells.Flow cytometry cell counting was used to test apoptosis rate.Results MiR-30d-5p had great relationship with Beclin1.QPCR testing miR-30d-5p had high expression in oral squamous cell carcinoma,Beclin1 had low expression,and there was negative correlation(P<0.05).Transfection miR-30d-5p could improve the increase of TSCC1,and inhibit TSCC1 cell apoptosis.MiR-30d-5p inhibitors could inhibit TSCC1 cell proliferation,improve TSCC1 cell apoptosis(P<0.05).After the lentiviral transfection TSCC1 through using Lenti-sh-Beclin,TSCC1 cell proliferation decreased,and apoptosis increased(P<0.05).Conclusion MiR-30d-5p can induce the proliferation of TSCC1 cells,and suppress TSCC1 cells apoptosis through the regulation of Beclin1.

关 键 词：人口腔鳞癌细胞 miR-30d-5p 细胞增殖 细胞凋亡 

分 类 号：R78[医药卫生—口腔医学]