拟南芥离体苗再生的分子机制研究进展  

Research progress on molecular mechanisms of de novo shoot regeneration in Arabidopsis thaliana

作　　者：代学焕 刘娜 王丽娟 向凤宁[1] 刘振华[1] DAI Xuehuan;LIU Na;WANG Lijuan;XIANG Fengning;LIU Zhenhua(Key Laboratory of Plant Development and Environmental Adaptation Biology,Ministry of Education,College of Life Sciences,Shandong University,Qingdao,Shandong 266237,China)

机构地区：[1]山东大学生命科学学院,植物发育与环境适应生物学教育部重点实验室,山东青岛266237

出　　处：《植物生理学报》2020年第8期1737-1744,共8页Plant Physiology Journal

基　　金：国家转基因重大专项(2018ZX0800914B);山东省自然科学基金重大基础研究项目(ZR2018ZC0334);国家转基因生物新品种培育重大专项(2016ZX08010002-009);中国博士后科学基金(2019-M662331);国家重点研发计划(2016YFD0101900);国家自然科学基金(31770317和31270328)。

摘　　要：植物细胞具有全能性,在离体条件下,植物体细胞可通过干细胞功能的获得、干细胞中心(stem cell niche)重建和芽尖分生组织形成不断地再生植株。随着分子生物学的发展,人们对植株再生的认识逐渐从培养体系优化深入到分子水平,多个再生相关的重要功能基因已经被挖掘,逐渐形成了以植物干细胞关键基因WUS(WUSCHEL)为核心的离体苗再生调控网络。本文主要综述了拟南芥离体苗再生的主要细胞学事件及分子调控机制的最新研究进展,并展望了未来植物再生机制研究的可能方向。Plant cells are totipotent.In vitro,plant somatic cells can continuously regenerate new plants through acquisition of stem cell function,stem cell niche reestablishment and shoot apical meristem formation.Based on the advances of molecular biology,studies of plant regeneration gradually focus on the underlying molecular mechanisms.A number of important genes involved in shoot regeneration have been discovered,and a regulatory network has been formed,in which WUS(WUSCHEL),a key stem cell related gene,is located in the center.Here,the latest advances of the main cytological events and molecular mechanisms of shoot regeneration in Arabidopsis thaliana are reviewed,and the possible research directions of plant regeneration are introduced.

关 键 词：细胞全能性 干细胞中心重建 离体苗再生 WUSCHEL 

分 类 号：Q945[生物学—植物学]