重组干酪乳杆菌喷雾干燥制剂制备工艺的优化  

Optimization of preparation process for spray-dried recombinant Lactobacillus casei

作　　者：张连妹 陈小燕 白永飞 李颖 蔡昌福 李树东[2] 刘欢欢 李岩 孙晓莹 杨雨晴 侯喜林[2] 余丽芸[1] ZHANG Lian-mei;CHEN Xiao-yan;BAI Yong-fei;LI Ying;CAI Chang-fu;LI Shu-dong;LIU Huan-huan;LI Yan;SUN Xiao-ying;YANG Yu-qing;HOU Xi-lin;YU Li-yun(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang Province,China;不详)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,黑龙江大庆163319 [2]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《中国生物制品学杂志》2020年第9期1037-1041,共5页Chinese Journal of Biologicals

基　　金：省高校产业化前期培育项目(1253CGZH14)。

摘　　要：目的优化重组干酪乳杆菌喷雾干燥制剂的制备工艺。方法以乳清蛋白为悬浮基质与重组干酪乳杆菌混匀,进行喷雾干燥,通过单因素试验确定乳清蛋白的添加量(10%、20%、30%、40%、50%、60%、70%、80%、90%)及进风温度(60、80、100、120、140、160、180℃)、给气量(65%、70%、75%、80%、85%、90%、95%、100%)和进料速度(2.5、5.0、7.5、10.0、12.5、15.0 mL/min)等喷雾干燥工艺参数;以活菌数和存活率为指标,通过正交试验进一步优化工艺参数。同时检测最适工艺制备的重组干酪乳杆菌喷雾干燥制剂的质量指标和贮藏稳定性。结果最适乳清蛋白添加量为20%,最适工艺参数:进风温度为120℃,给气量为75%,进料速度为10 mL/min。喷雾干燥制剂相关的质量指标均符合要求,在4℃真空条件下贮藏稳定性最好。结论优化了重组干酪乳杆菌喷雾干燥制剂的制备工艺,为其产业化生产奠定了基础。Objective To optimize the preparation process for spray-dried recombinant Lactobacillus casei.Methods Recombinant L.casei was mixed with whey protein as a suspension matrix for spray drying,while the addition amount of whey protein(10%,20%,30%,40%,50%,60%,70%,80%and 90%),inlet air temperature(60,80,100,120,140,160 and 180℃),air input(65%,70%,75%,80%,85%,90%,95%and 100%)and charging rate(2.5,5.0,7.5,10.0,12.5 and 15.0 mL/min)were optimized by single factor test.Orthogonal experiments were used to further optimize the process parameters using viable count and survival rate as indicators.The quality and storage stability of spray-dried recombinant L.casei prepared under the optimal condition were tested.Results The optimal addition amount of whey protein,inlet air temperature,air input and the charging rate were 20%,120℃,75%and 10 mL/min respectively.The relevant quality indicators of the spray-dried preparation met the relevant requirements,while the stability was satisfactory after storage at 4℃in vacuum.Conclusion The preparation process for spray-dried recombinant L.casei was optimized,which laid a foundation of industrial production of the spray-dried preparation.

关 键 词：重组干酪乳杆菌 喷雾干燥 工艺参数 正交试验 

分 类 号：Q812[生物学—生物工程]