银桦花醇提物成分预试及其抗氧化活性  

Preliminary Chemical Analysis AndAntioxidant Activity of the Ethanol Extract From Flower of Grevillea robusta

作　　者：林大都[1] 张勇[1] 翟明[1] 李兰芳[1] 李俊芳[1] 张声源[1] LIN Dadu;ZHANG Yong;ZHAI Ming;LI Lanfang;LI Junfang;ZHANG Shengyuan(Medical College,Jiaying University,Meizhou,Guangdong 514000)

机构地区：[1]嘉应学院医学院,广东梅州514000

出　　处：《北方园艺》2020年第19期113-117,共5页Northern Horticulture

基　　金：国家自然科学基金青年基金资助项目(81703662);广东省中医药局科研资助项目(20161251);广东省科技计划资助项目(2014A020221061,2015A090905019);嘉应学院自然科学研究项目一般资助项目(2015KJY11,2019KJY09)。

摘　　要：以银桦花为试材,采用化学反应鉴别法分析银桦花乙醇提取物的成分类型,并通过水杨酸法、铁氰化钾还原法、DPPH法和ABTS法评价银桦花不同极性部位的体外抗氧化活性,以期提高银桦花更为广泛的应用价值。结果表明:银桦花乙醇提取物中可能含有黄酮类、酚类、蒽醌类、强心苷和香豆素内酯类成分;不同极性部位均具有一定的抗氧化活性,且呈一定量效关系,其中水杨酸法、铁氰化钾还原法和ABTS法中乙酸乙酯部位表现出最强的抗氧化活性,而DPPH法中石油醚部位对DPPH自由基的清除能力最强。Chemical reaction was applied to analyze the component type of theethanol extract from the flower of Grevillea robusta.Meanwhile,the antioxidant activity of different polarity partsofits flower were estimated by methods of salicylic acid,potassium ferricyanide reduction,2-diphenyl-1-picrylhydrozyl(DPPH)and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS).The results showed that theethanol extract contained chemical constituents such as flavonoid,phenols,anthraquinones,cardiac glycoside and coumarins.The different polarity parts in extracts with ethanol from the flower of Grevillea robusta all had antioxidant activity.The ethyl acetate extract showed the strongest antioxidant activity in the tests of salicylic acid method,potassium ferricyanide reduction method and ABTS method.The petroleum etherextract showed the strongest scavenging activity in DPPH method than other extracts.

关 键 词：银桦花 乙醇提取物 成分预试 抗氧化活性 

分 类 号：S687[农业科学—观赏园艺]