检测活性硫物种小分子荧光探针的研究进展  

Progress of Small Molecular Fluorescent Probes for Detecting Active Sulfur Species

作　　者：刘喜灵 张淑平[1] LIU Xi-ling;ZHANG Shu-ping(College of Science,University Of Shanghai For Science And Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学理学院,上海200093

出　　处：《广州化学》2020年第4期41-48,共8页Guangzhou Chemistry

摘　　要：总结了近两年来检测生物硫醇、H2S以及SO2衍生物荧光探针的研究现状,着重讨论了探针设计的传感机制、传感性能和生物应用方面的特征。检测生物硫醇方面,主要介绍了涉及多反应位点的多通道探针用于区分谷胱甘肽(GSH)、半胱氨酸(Cys)和同型半胱氨酸(Hcy);检测H2S方面,重点介绍了基于叠氮基还原和亲核反应这两种机制设计的探针;检测SO2衍生物方面,主要总结了根据迈克尔加成法和醛的加成机制设计的探针。这为构建新型荧光探针以研究活性硫物种的生理和病理作用奠定了基础。This paper summarizes the research status of the fluorescent probe for the detection of biothiol, H2S and SO2 derivatives in the past two years. The sensing mechanism of the probe design, the sensing performance, and the characteristics of biological applications are discussed. A multi-channel probe for the detection of biothiols was introduced. It has multiple reaction sites to distinguish GSH, Hcy and Cys. Two main strategies for H2S detection in molecular probe development are introduced, including azide reduction and nucleophilic attack reactions. For the detection of SO2 derivatives, the two mechanisms are mainly summarized, including Michael addition reaction and nucleophilic reaction with aldehyde. This has laid an important foundation for the construction of new fluorescent probe to study the physiological and pathological effects of active sulfur species.

关 键 词：活性硫 荧光探针 传感机制 

分 类 号：O622.1[理学—有机化学]