RNF187在肝癌组织中的表达及其与预后的关系  

Association of expression of ring finger protein 187 with tumor metastasis and clinical prognosis inhepatocellular carcinoma

作　　者：唐荣[1] 郑林媚[2] 张震生[1] 武金才[1] 郑进方[1] 陈良[1] 丁驿超 陈平平[1] 章家超 曾勇超 TANG Rong;ZHENG Lin-mei;ZHANG Zhen-sheng;WU Jin-cai;ZHENG Jin-fang;CHEN Liang;DING Yi-chao;CHEN Ping-ping;ZHANG Jia-chao;ZENG Yong-chao(Department of Hepatopancreatobiliary Surgery,Hainan Provinicial People's Hospital,Haikou 570311,China;Obstetrical department,Hainan Provinicial People's Hospital,Haikou 570311,China)

机构地区：[1]海南省人民医院/海南医学院附属海南医院肝胆胰外科,海南海口570311 [2]海南省人民医院/海南医学院附属海南医院产科,海南海口570311

出　　处：《海南医学院学报》2020年第20期1531-1536,共6页Journal of Hainan Medical University

基　　金：海南省重点研发计划(ZDYF2019120);国家自然科学基金项目(81660489、81260367、81160310);海南省自然科学基金项目(819QN356)。

摘　　要：目的:探讨RNF187在肝癌组织中的表达及其与预后的关系。方法:应用实时荧光定量PCR(qRT-PCR)与蛋白质印迹(Western blot)检测肝细胞癌(hepatocellular carcinoma,HCC)患者肿瘤组织和癌旁组织中RNF187的表达。免疫组化检测HCC组织标本中RNF187的表达,并分析其与肿瘤的临床病理特征及预后的关系。结果:肝癌组织中RNF187的mRNA和蛋白表达水平均明显高于癌旁组织,且在有子灶或血管癌栓的HCC癌组织中的表达明显高于无子灶或血管癌栓患者的癌组织(P均<0.05)。RNF187高表达组术后5年生存率明显低于低表达组(P均<0.05);Cox分析显示RNF187高表达是肝癌术后生存(HR=1.313,95%CI=1.128~1.537,P<0.001)的独立危险因素。结论:RNF187在肝癌组织中的高表达与肿瘤复发转移及预后密切相关,或将成为肝癌复发转移及预后预测的分子指标。Objective To investigate the expression of RNF187 in hepatocellular carcinoma(HCC)and itsrelationship with prognosis.MethodsThe mRNA and protein expressions in hepatocellular carcinoma and tumor-adjacent tissues of HCC patientswere determined by qRT-PCR and Western blot analysis,and RNF187 expressions in HCC were detected by immunohistochemistry.The relationship of RNF187 expression with clinicopathologic factors and prognosis in HCC patients were analyzed.ResultsRNF187 expressed highly in HCC tissues compared with the corresponding adjacent liver tissues both in mRNA and protein level,and significantly higher in HCC tissue from patients with intrahepatic metastases or vascular invasion than those in HCC tissue from patients without intrahepatic metastases and vascular invasion(all P<0.05).The 5-year survival rate of the high expression group was lower than that of the low expression group(P<0.05).Cox regression analysis revealed that high RNF187 expression was independent risk factor for postoperativesurvival(HR=1.313,95%CI=1.128-1.537,P<0.001).ConclusionHigh expression of RNF187in HCC is closely related to tumor prognosis,which may become a new index to evaluate the clinical diagnosis and prognosis of liver cancer.

关 键 词：肝细胞癌 RNF187 临床病理特征 预后 

分 类 号：R735.7[医药卫生—肿瘤]