白蒺藜皂苷对2型糖尿病大鼠视网膜的保护作用及TrkB信号通路的影响  

Protective Effect of Tribulus Terrestris Saponins on Retina and TrkB Signaling Pathway in Type 2 Diabetic Rats

作　　者：赵艳霞[1] 王锋 王爽[1] ZHAO Yan-xia;WANG Feng;WANG Shuang(Department of Ophthalmology,Harbin 242 Hospital,Harbin 150066,China;Department of Radiology,Harbin 242 Hospital,Harbin 150066,China)

机构地区：[1]哈尔滨二四二医院眼科,哈尔滨150066 [2]哈尔滨二四二医院放射线科,哈尔滨150066

出　　处：《解放军医药杂志》2020年第10期24-28,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：黑龙江省卫生计生委科研课题(2017407)。

摘　　要：目的探讨白蒺藜皂苷对2型糖尿病大鼠视网膜的保护作用及TrkB信号通路的影响。方法清洁级SD大鼠100只分为对照组、模型组、二甲双胍组、白蒺藜皂苷低、高剂量组,每组20只。模型组、二甲双胍组、白蒺藜皂苷低、高剂量组腹腔注射60 mg/kg链脲佐菌素诱导糖尿病视网膜病变模型;建模成功后,二甲双胍组给予二甲双胍20 mg/kg腹腔灌胃,白蒺藜皂苷低、高剂量组分别给予白蒺藜皂苷20、40 mg/kg腹腔灌胃,对照组和模型组给予等体积的生理盐水。测定5组大鼠血糖及视网膜组织伊凡斯兰渗透量、神经节细胞凋亡水平、脑源性神经营养因子(BDNF)和p-TrkB mRNA及蛋白水平。结果与对照组比较,模型组血糖、视网膜组织伊凡斯兰渗透量、神经节细胞凋亡水平升高,BDNF、p-TrkB mRNA和蛋白水平降低(P<0.05)。与模型组比较,二甲双胍组、白蒺藜皂苷低、高剂量组血糖、视网膜组织伊凡斯兰渗透量水平、神经节细胞凋亡水平降低,BDNF、p-TrkB mRNA和蛋白水平升高(P<0.01)。与二甲双胍组比较,白蒺藜皂苷低、高剂量组血糖、视网膜组织伊凡斯兰渗透量、神经节细胞凋亡水平升高,BDNF、p-TrkB mRNA和蛋白水平降低(P<0.05)。白蒺藜皂苷高剂量组血糖、视网膜组织伊凡斯兰渗透量、神经节细胞凋亡水平低于白蒺藜皂苷低剂量组,BDNF、p-TrkB mRNA和蛋白水平高于白蒺藜皂苷低剂量组(P<0.01)。结论白蒺藜皂苷能降低2型糖尿病大鼠血糖水平,对视网膜具有保护作用,其机制与白蒺藜皂苷能促进2型糖尿病大鼠视网膜BDNF、p-TrkB mRNA和蛋白高表达,进而激活TrkB信号通路相关。Objective To investigate the protective effect of Tribulus terrestris saponins on retina and TrkB signaling pathway in rats with type 2 diabetes mellitus(T2DM).Methods A total of 100 clean SD rats were divided into control group,model group,Metformin group,low and high-dose Tribulus terrestris saponins groups with 20 rats in each group.Model group,Metformin group,low and high-dose Tribulus terrestris saponins groups received an intraperitoneal injection of 60 mg/kg Streptozotocin to induce diabetic retinopathy model.After successful modeling,the Metformin group was given an intraperitoneal injection of Metformin(20 mg/kg)by gavage,while low and high-dose Tribulus terrestris saponins groups were given an intraperitoneal injection of Tribulus terrestris saponins(20 and 40 mg/kg respectively)by gavage.The control group and model group were given equal volumes of normal saline.Blood sugar and Ivansland permeability of rats'retinal tissue were measured,and the apoptotic level of retinal cells was detected.Retinal brain-derived neurotrophic factor(BDNF)and p-TrkB mRNA and protein levels of the rats were measured.Results Compared with the control group,the blood sugar,Ivanslam permeability of retinal tissue and apoptotic level of ganglion cells in the model group were increased,while the levels of BDNF,and p-TrkB mRNA and protein were decreased(P<0.05).Compared with the model group,the blood sugar,Ivanslam permeability of retinal tissue and apoptotic level of ganglion cells in the Metformin group and low-dose and high-dose Tribulus terrestris saponins groups were decreased,while the levels of BDNF,p-TrkB mRNA and protein were increased(P<0.01).Compared with Metformin group,the blood sugar,Ivanslam permeability of retinal tissue and apoptotic level of ganglion cells in low-dose and high-dose Tribulus terrestris saponins groups were increased,whereas the levels of BDNF,p-TrkB mRNA and protein were decreased(P<0.05).The blood sugar,Ivanslam permeability of retinal tissue and apoptotic level of ganglion cells in high-dose t

关 键 词：糖尿病 2型 糖尿病视网膜病变 白蒺藜皂苷 血糖 视网膜神经元 脑源性神经营养因子 肌球蛋白受体激酶B 大鼠 Sprague-Dawley 

分 类 号：R-332[医药卫生] R587.26