脂肪来源干细胞培养基对瘢痕成纤维细胞增殖及胶原合成的影响  

Effects of adipose-derived stem cells medium on the hypertrophic scar fibroblasts proliferation and collagen synthesis

作　　者：胡华婷 王巧稚 李静婷 曾永秋 刘广益 HU Hua-ting;WANG Qiao-zhi;LI Jing-ting;ZENG Yong-qiu;LIU Guang-yi(Morphology Laboratory,Basic Medical College,Basic Medical College,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Histology and Embryology Teaching and Research Section,Basic Medical College,Basic Medical College,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Experimental Center,Nursing College,Basic Medical College,Southwest Medical University,Luzhou 646000,Sichuan Province,China;Biology Teaching and Research Section,Basic Medical College,Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学基础医学院形态学实验室,四川泸州646000 [2]西南医科大学基础医学院组胚教研室,四川泸州646000 [3]西南医科大学护理学院实验中心,四川泸州646000 [4]西南医科大学基础医学院生物教研室,四川泸州646000

出　　处：《中国临床药理学杂志》2020年第19期3091-3094,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨脂肪来源干细胞(ADSCs)培养基对瘢痕成纤维细胞增殖与胶原合成的影响及其潜在机制。方法取正常成纤维细胞(NFs)和瘢痕成纤维细胞(HSFs)分别用成纤维细胞培养基和添加ADSCs条件培养基的成纤维细胞培养基培养,实验分为A,B,C,D组。A组给予NFs成纤维培养基(FM)处置;B组给予NFs脂肪干细胞条件培养基(ADSCs+FM)处置;C组给予HSFs FM处置;D组给予HSFs ADSCs+FM处置。用噻唑蓝法检测细胞增殖率,用免疫印迹法检测胶原合成和相关通路蛋白的表达情况。结果C组与D组培养48 h的细胞增殖率分别为(5.03±1.77)%和(1.54±0.71)%,差异有统计学意义(P<0.01)。C组与D组的Ki-67蛋白相对表达量分别为0.35±0.01和0.14±0.01,p-p38蛋白相对表达量分别为0.37±0.02和0.21±0.01,α-SMA分别为1.58±0.09和0.93±0.07,Ⅰ型胶原分别为0.61±0.02和0.24±0.01,Ⅲ型胶原分别为0.44±0.05和0.29±0.03,差异均有统计学意义(均P<0.01)。结论ADSCs条件培养基抑制了瘢痕成纤维细胞增殖,其可能通过调控p38蛋白的磷酸化,从而缓解瘢痕成纤维细胞的增殖与纤维化。Objective To investigate the effects of adipose-derived stem cells(ADSCs)medium on proliferation and collagen synthesis of scar fibroblasts and its potential mechanism.Methods Normal fibroblasts(NFS)and hypertrophic scar fibroblasts(HSFs)were cultured with fibroblast medium and fibroblast medium supplemented with ADSCs conditioned medium respectively.The experiment were divided into A,B,C and D group.A group was NFS treated with fibroblast medium(FM);B group was NFS treated with ADSCs+FM;C group was HSFs treated with FM;and D group was HSFs treated with ADSCs+FM.Methyl thiazolyl tetrazolium method was used to detect cell proliferation rate.Western blot was used to detect the expressions of collagen synthesis and related pathway proteins.Results The 48 h cell proliferation rates of C group and D group were(5.03±1.77)%and(1.54±0.71)%with significant difference(P<0.01).In C and D group,the relative expressions of Ki-67 protein were 0.35±0.01 and 0.14±0.01,the relative expressions of p-p38 protein were 0.37±0.02 and 0.21±0.01,theα-SMA were 1.58±0.09 and 0.93±0.07,the collagenⅠwere 0.61±0.02 and 0.24±0.01,the collagenⅢwere 0.44±0.05 and 0.29±0.03,the differences were all statistically significant(all P<0.01).Conclusion ADSCs conditioned medium inhibits scar fibroblast proliferation,which may alleviate the proliferation and fibrosis of scar fibroblasts by regulating the phosphorylation of p38 protein.

关 键 词：脂肪来源干细胞 瘢痕成纤维细胞 增殖 胶原合成 P38信号通路 

分 类 号：R363[医药卫生—病理学]