Genetic diversity and population structure of Camellia huana(Theaceae),a limestone species with narrow geographic range,based on chloroplast DNA sequence and microsatellite markers  

作　　者：Shuang Li Shang-Li Liu Si-Yu Pei Man-Man Ning Shao-Qing Tang 

机构地区：[1]Guangxi Key Laboratory of Rare and Endangered Animal Ecology,College of Life Science,Guangxi Normal University,Guilin,China [2]Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,Guangxi Normal University,Guilin,China [3]Longtan Nature Reserve Management Center,Hechi,China [4]College of Life Science,Guangxi Normal University,Guilin,Guangxi 541006,China

出　　处：《Plant Diversity》2020年第5期343-350,共8页植物多样性（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(grant number 31760088);Biodiversity Survey,Observation and Assessment Program of the Ministry of Ecology and Environment of China(grant number 837208).

摘　　要：Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces,China.We used one chloroplast DNA(cpDNA)fragment and 12 pairs of microsatellite(simple sequence repeat;SSR)markers to assess the genetic diversity and structure of 12 C.huana populations.A total of 99 alleles were detected for 12 polymorphic loci,and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA.C.huana populations showed a low level of genetic diversity(n=8,Hd=0.759,Pi=0.00042 for cpDNA,NA=3.931,HE=0.466 for SSRs),but high genetic differentiation between populations(FST=0.2159 for SSRs,FST=0.9318 for cpDNA).This can be attributed to the narrow distribution and limestone habitat of C.huana.STRUCTURE analysis divided natural C.huana populations into two groups,consistent with their geographical distribution.Thus,we suggest that five natural C.huana populations should be split into two units to be managed effectively.

关 键 词：Camellia huana Genetic diversity Genetic structure Conservation implications 

分 类 号：S571.1[农业科学—茶叶生产加工]