葡萄糖氧化酶和嗜酸乳杆菌与小檗碱配伍对大肠杆菌生长的抑制作用  

Inhibition Effects of Glucose Oxidase and Lactobacillus acidophilus and Berberine on the Proliferation of Escherichia coli

作　　者：陈景岩 耿启泉 常娟[1] 王平[1] 尹清强[1] 刘超齐 李茂龙 朱群 卢富山 CHEN Jing-yan;GENG Qi-quan;CHANG Juan(College of Animal Science&Technology,Henan Agricultural University,Zhengzhou,Henan 450046;Weihui Agricultural and Rural Bureau of Henan Province,Weihui,Henan 453100)

机构地区：[1]河南农业大学动物科技学院,河南郑州450046 [2]河南省卫辉市农业农村局,河南卫辉453100 [3]河南德邻生物制品有限公司,河南新乡453000 [4]河南普爱饲料股份有限公司,河南周口466000

出　　处：《安徽农业科学》2020年第20期95-98,122,共5页Journal of Anhui Agricultural Sciences

基　　金：河南省重大科技专项(171100110500);新乡市重大科技专项(ZD19005)。

摘　　要：为了获得抗生素的最佳替代品,采用单因素和多因素设计开展了葡萄糖氧化酶(GOD)和嗜酸乳杆菌与小檗碱(BBR)配伍对大肠杆菌生长抑制作用的研究。单因素试验结果表明,葡萄糖氧化酶、嗜酸乳杆菌、小檗碱和金霉素均对大肠杆菌生长有显著的抑制作用(P<0.05),并且随着浓度的增加其抑制作用逐步增强。多因素试验结果表明,选取0.0050%GOD和1×105 CFU/mL嗜酸乳杆菌与0.0015%BBR组合对大肠杆菌的抑制作用与75~150 mg/kg金霉素的抑菌作用相当。通过该研究获得了一种新的抗生素替代品,为畜产品安全生产提供了保障。In order to obtain the optimal substitute of antibiotics,the inhibition effects of combination of glucose oxidase(GOD)and Lactobacillus acidophilus with berberine(BBR)on the growth of Escherichia coli were studied by single-factor and multi-factor experiments.The results of single-factor experiment showed that GOD,L.acidophilus,BBR and aureomycin individually had significant inhibitory effects on the proliferation of E.coli(P<0.05),and the antibacterial activity gradually increased with increasing of their concentrations.The multi-factor experiments showed that the combination of 0.0050%GOD,1×105 CFU/mL L.acidophilus and 0.0015%BBR had the same inhibitory effect on E.coli growth with 75-150 mg/kg aureomycin.A new antibiotic substitute was obtained,which provided guarantee for the safe production of livestock products.

关 键 词：葡萄糖氧化酶 嗜酸乳杆菌 小檗碱 金霉素 抑菌作用 

分 类 号：S816.7[农业科学—饲料科学]