尿干化学法和离心沉渣镜检法检测穿刺羊水母体细胞污染的应用  

Application of Detecting Maternal Cell Contamination in Amniocentesis with Drop-Stick Test and Centrifugal Sediment Microscopy

作　　者：林俊伟[1] 章钧[1] 侯红瑛[1] 叶燕绸[1] 詹龙生 刘城 LIN Jun-wei;ZHANG Jun;HOU Hong-ying;YE Yan-chou;ZHAN Long-sheng;LIU Cheng(Department of Obstetrics and Gynecology,the Third Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院妇产科,广州510630

出　　处：《现代检验医学杂志》2020年第5期90-92,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨一种快速简便鉴别羊水母血污染的方案。方法选取30例2019年6~10月羊水穿刺样本,检测羊水及母血DNA,将母血DNA污染量换算对应体积全血量建立羊水母血DNA污染梯度模型,运用尿干化学法及离心沉渣镜检法检测母血污染情况。结果羊水母血DNA污染率为5%,10%,20%和30%,尿干化学法检出率分别为16.67%,60%,100%和100%;离心沉渣镜检法检出率分别为60%,100%,100%和100%。结论尿干化学法及离心沉渣镜检法联合检测可更简便快捷地进行羊水母血污染鉴定,检出率与母血DNA污染率呈正比关系。Objective To establish a quick and simple method to identify the maternal cell contamination in amniotic fluid.Methods 30 samples of amniotic fluid puncture samples from June to October 2019 were selected to detect the amniotic fluid and maternal blood DNA.The contamination amount of maternal blood DNA was converted into the corresponding volume and total blood volume to establish the gradient model of amniotic fluid blood DNA contamination.The Drop-stick test method and centrifugal sediment microscopy were used to detect the contamination of maternal blood.Results The contamination rate of maternal DNA was 5%,10%,20%and 30%,and the detection rate of drop-stick test was 16.67%,60%,100%and 100%.The detection rate of centrifuge sediment microscopy was 60%,100%,100%and 100%.Conclusion Combined detection of dropstick test and sediment microscopy can more easily and quickly identify the maternal cell contamination in amniotic fluid.The detection rate is in direct proportion to the DNA contamination rate of mother blood.

关 键 词：尿干化学法 离心沉渣镜检法 母体细胞污染 

分 类 号：R446.19[医药卫生—诊断学]