埃吉类芽孢杆菌SWL-W8的鉴定及其对白菜软腐病的生物防治效果  

Identification of a strain Paenibacillus elgii SWL-W8 and its biocontrol effect against soft rot of Chinese cabbage

作　　者：卢美欢 李利军[2] 马英辉[2] 陈雅寒[1] 安德荣[1] LU Meihuan;LI Lijun;MA Yinghui;CHEN Yahan;AN Derong(College of Plant Protection,State Key Laboratory of Crop Stress Biology,Northwest A&F University,Yangling 712100,Shaanxi Province,China;Shaanxi Institute of Microorganism,Xi’an 710043,China)

机构地区：[1]西北农林科技大学植物保护学院,旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]陕西省微生物研究所,西安710043

出　　处：《农药学学报》2020年第5期791-800,共10页Chinese Journal of Pesticide Science

基　　金：陕西省科技厅重点研发计划(2020NY-046);西安市科技局农业创新计划(2017050NC/NY009(4));陕西省科学院科技计划项目(2015K-05).

摘　　要：从云南省腾冲市温泉中筛选到1株对白菜软腐病病原菌Pectobacterium carotovorum subsp.carotovorum具有较好拮抗作用的菌株SWL-W8,对该菌株进行了鉴定和抑菌活性测试,并研究了其对白菜软腐病的防治效果。结果显示:通过菌株形态、BIOLOG及16S rDNA鉴定SWL-W8菌株为埃吉类芽孢杆菌Paenibacillus elgii,并发现该菌株对多种病原菌有较好的抑制效果。利用Nanopore测序技术对SWL-W8进行全基因组测序,显示该菌株含有表面活性素(surfactin)、聚酮化合物(polyketide)、枯草杆菌蛋白酶(subtilisin)、几丁质酶(chitinase)、杆菌素(bacillibactin)、杀镰孢菌素(fusaricidin)、羊毛硫抗生素(elgicin)等脂肽类、肽聚糖和聚酮类抗性化合物的基因,揭示了其可能产生的次生代谢产物及潜在的生防作用机制。菌株SWLW8在土壤中能较好地定殖,菌悬液浓度为1.0×10^6 cfu/mL时对白菜软腐病的最高预防作用防效为79.7%,治疗作用防效为64.4%,且能促进白菜种子萌发和生长,具有很好的开发应用前景。The bacterial strain SWL-W8 with good antagonism against the soft rot pathogen Pectobacterium carotovorum subsp.carotovorum was isolated from the hot springs at Tengchong City in Yunnan Province.The purpose of this study was to identify the strain,evaluate its antibacterial ability,and study the control effect of the strain against soft rot of Chinese cabbage.Based on its morphological characterisitcs,the BIOLOG identification results,and the annalysis of its 16 S rDNA sequence,strain SWL-W8 was identified to be a member of Paenibacillus elgii.It exhibited inhibitory activities against several pathogens,including the pathogenic P.carotovorum that causes bacterial soft rot in plants.Whole genome sequencing analysis of strain SWL-W8 using nanopore sequencing technique indicated that the strain harbors encoding genes of lipopeptides,peptidoglycans,and polyketide resistant compounds such as surfactin,polyketide,subtilisin,chitinase,bacillibactin,fusaricidin and elgicin.SWL-W8 could easily colonize in soil.In pot experiments,the protection and cure efficacy of SWL-W8 bacterial suspension at a concentration of 1.0×10^6 cfu/mL against soft rot of Chinese cabbage were 79.7%and 64.4%,respectively.In addition,SWL-W8 bacterial suspension promoted the seeds germination and Chinese cabbage growth.In conclusion,strain SWL-W8 is a potential candidate for further development in plant disease control.

关 键 词：埃吉类芽孢杆菌 菌株鉴定 全基因组测序 白菜软腐病 生物防治 作用机制 

分 类 号：S482.292[农业科学—农药学] S481.9[农业科学—植物保护]