淫羊藿总黄酮清除DPPH自由基的紫外-可见吸收光谱研究  

Study on the UV-Vis Absorption Spectra of Scavenging DPPH Free Radicalsby Total Flavonoids of Epimedium

作　　者：姚金龙 YAO Jin-long(Department of Pharmaceutical Engineering,Jiangxi Medical School,Nanchang 330200,Jiangxi,China)

机构地区：[1]江西省医药学校制药工程系,江西南昌330200

出　　处：《医学信息》2020年第20期164-166,共3页Journal of Medical Information

摘　　要：目的研究淫羊藿总黄酮的提取、分离及纯化工艺,建立紫外-可见吸收光谱滴定法,评价淫羊藿总黄酮清除DPPH自由基的抗氧化活性。方法通过保持其它条件不变,分别考察浸提温度、料液比和乙醇浓度对提取液中淫羊藿总黄酮含量的影响,设计三因素三水平的正交实验,提取温度60℃~80℃,乙醇浓度50%~70%,料液比1:35~1:15,做3次平行实验,优化淫羊藿总黄酮的最佳提取工艺条件。结果温度70℃、60%乙醇浓度和1:25料液比是最优提取工艺条件,淫羊藿精提液中总黄酮质量浓度为10.15μg/ml,含量为31.72%。光谱滴定法结果表明,淫羊藿总黄酮的质量浓度≥0.002 mg/ml时,清除率达79.17%。结论淫羊藿总黄酮对DPPH自由基有很好的清除能力,抗氧化活性强。Objective To study the extraction,separation and purification process of total flavonoids of epimedium,and establish a UV-Vis absorption spectrometric titration method to evaluate the antioxidant activity of total flavonoids of epimedium in scavenging DPPH free radicals.Methods By keeping other conditions unchanged,the effects of extraction temperature,material-to-liquid ratio and ethanol concentration on the total flavonoids content of epimedium in the extract were investigated respectively.An orthogonal experiment with three factors and three levels was designed,and the extraction temperature was 60℃~80℃,the ethanol concentration is 50%~70%,the material-liquid ratio is 1:35~1:15,three parallel experiments are performed to optimize the optimal extraction process conditions of the total flavonoids of epimedium.Results Temperature 70℃,60%ethanol concentration and 1:25 solid-liquid ratio were the optimal extraction conditions.The mass concentration of total flavonoids of epimedium extract was 10.15μg/ml and the content was 31.72%.The results of spectrometric titration showed that when the mass concentration of total flavonoids of epimedium≥0.002 mg/ml,the clearance rate reached 79.17%.Conclusion The total flavonoids of epimedium had good scavenging ability to DPPH free radicals and strong antioxidant activity.

关 键 词：淫羊藿总黄酮 紫外-可见吸收光谱 DPPH自由基 抗氧化 

分 类 号：R285[医药卫生—中药学] TS201.2[医药卫生—中医学]