Meis1与VEGFR-2在早期肾癌组织中的表达及其临床意义  

Expression of VEGFR-2 and Meis1 in the early stage of kidney cancers and its clinical significance

作　　者：万邦贝 刘波 王兆兴 雷青青 吕蔡[1] WAN Bangbei;LIU Bo;WANG Zhaoxing;LEI Qingqing;LÜCai(Department of Urology,Haikou Hospital Affiliated to Xiangya School of Medicine,Central South University,Haikou 570208;Reproductive Medical Center,Maternity&Child Health Hospital of Hainan Province,Haikou 570206;Department of Ophthalmology,Eye Hospital of Wenzhou Medical University,Wenzhou Zhejiang 325000,China)

机构地区：[1]中南大学湘雅医学院附属海口医院泌尿外科,海口570208 [2]海南省妇幼保健院生殖医学中心,海口570206 [3]温州医科大学附属眼视光医院眼科,浙江温州325000

出　　处：《中南大学学报（医学版）》2020年第9期1061-1067,共7页Journal of Central South University ：Medical Science

基　　金：海南省卫健委科研项目(2018-123)。

摘　　要：目的:探讨髓系亲嗜性白血病病毒整合位点1(myeloid ecotropic viral integration site 1,Mesi1)及血管内皮生长因子受体2(vascular endothelial growth factor receptor 2,VEGFR-2)在人早期肾癌中表达的临床意义及相关性。方法:收集中南大学湘雅医学院附属海口医院2005年4月至2018年9月间手术切除后的肾癌及癌旁组织标本,其中石蜡组织80对,新鲜冻存组织15对。利用real-time PCR及免疫组织化学技术分别检测肾癌组织中Meis1和VEGFR-2的mRNA和蛋白质表达水平,并分析其与患者的临床病理学参数及预后的相关性。结果:肾癌组织中Meis1和VEGFR-2的mRNA和蛋白质表达水平均较癌旁组织低,差异均有统计学意义(均P<0.01),并且Meis1与VEGFR-2在表达量上呈正相关(r=0.681,P<0.01)。患者的临床病理学相关参数分析显示:Meis1表达的阳性率与肾癌的病理类型有关(P<0.01),Meis1和VEGFR-2表达的阳性率与肾癌患者性别、年龄、T分期等均无关,差异均无统计学意义(均P>0.05),但其与患者的预后明显有关(P<0.05)。Cox回归分析显示:Meis1是影响患者预后的独立因素(95%CI:0.08~1.85,P<0.05)。结论:早期肾癌组织中Meis1和VEGFR-2的mRNA和蛋白质表达水平明显降低,在肾癌中可能可作为肿瘤抑制因子来影响肾癌的发生、发展,Meis1可作为预测肾癌患者预后的生物标志物。Objective:To evaluate the expression of myeloid ecotropic viral integration site 1(Meis1)and vascular endothelial growth factor receptor 2(VEGFR-2)in early-stage kidney cancers and the clinical significance.Methods:The cancer tissues and the matched adjacent normal tissues in patients with kidney cancer,who received surgical treatment from April 2005 to September 2018 in the Haikou Hospital Affiliated to Xiangya School of Medicine,Central South University,were collected.The samples included 80 pairs of paraffin specimen,15 pairs of fresh cancer and the matched adjacent normal tissues from these patients.Real-time PCR and immunohistochemical method were used to detect the expression levels of Meis1 and VEGFR-2 m RNA and protein in kidney tissues and adjacent normal tissues,and the correlation of clinical pathology parameters and the prognosis were analyzed in the patients.Results:The expression levels of Meis1 and VEGFR-2 m RNA and protein in the renal carcinoma tissues were lower than those in the matched adjacent normal tissues(both P<0.01),and the expression levels of Meis1 were positively correlated with that of VEGFR-2(r=0.681,P<0.01).The analysis of relevant clinical-pathological parameters in the patients showed that:the expression positive rate of Meis1 was significantly related with the pathological type of renal cancer(P<0.01),while the positive rate of Meis1 and VEGFR-2 expression was not related with the gender,age,T stage of patients(all P>0.05),but it was significantly related with the prognosis in the patients(P<0.05).Cox regression analysis showed that:Meis1 was an independent factor for the prognosis of patients(P<0.05).Conclusion:The m RNA and protein expression levels of Meis1 and VEGFR-2 in the earlystage kidney cancer tissues are significantly decreased compared with those in the adjacent normal tissues.Meis1 may be served as a tumor suppressor to affect the occurrence and development of kidney cancer.Therefore,Meis1 may be used as a biomarker to predict the prognosis of patients with kidney c

关 键 词：髓系亲嗜性白血病病毒整合位点1 血管内皮生长因子受体2 肾癌 

分 类 号：R737.11[医药卫生—肿瘤]