枸杞酒多糖的提取、成分测定及其对酒精性肝病的影响研究  

Extraction and determination of polysaccharides from Lycium barbarum liquor and its effect on alcoholic liver disease

作　　者：赵嘉庆[1,2] 史春丽 王立英 何斌 赵巍 王浩[1] ZHAO Jiaqing;SHI Chunli;WANG Liying;HE Bin;ZHAO Wei;WANG Hao(College of Basic Medical,Ningxia Medical University,Yinchuan 750004,China;Institute of Medical Sciences of Ningxia Hui Autonomous Region,Yinchuan 750004,China;Ningxia Senmiao Goji Technology and Development Co.,Ltd.,Yinchuan 750000,China)

机构地区：[1]宁夏医科大学基础医学院,宁夏银川750004 [2]宁夏回族自治区医学科学研究所,宁夏银川750004 [3]宁夏森淼枸杞科技开发有限公司,宁夏银川750000

出　　处：《中国酿造》2020年第10期114-118,共5页China Brewing

基　　金：宁夏回族自治区科技支撑项目(2015BY033)。

摘　　要：以枸杞酒作为原料提取枸杞多糖,对其成分进行测定,并探讨其对酒精肝病的影响。将60只雄性C57BL/6小鼠随机分为对照组、模型组、对照+枸杞多糖组、模型+枸杞多糖组。测定各组小鼠血清丙氯酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)及内毒素(LPS)的含量;酶联免疫吸附实验(ELISA)检测小鼠血清和肝脏炎症因子的变化。结果表明,枸杞酒中提取出的多糖含量为1.2 g/L,其中矿物质元素有Ca、P、Mg、K、Na,分别为0.09%、0.69%、0.28%、3.92%、0.51%。其17种氨基酸中天冬氨酸(ASP)及谷氨酸(GLU)的含量较多,分别为7.25%及4.96%。与模型组相比,模型+枸杞多糖组ALT、AST及LPS含量明显降低(P<0.05),炎症细胞因子IL-1β、IL-6、TNF-α含量显著降低(P<0.05)。表明枸杞酒多糖可明显缓解酒精性肝损伤。Lycium barbarum(wolfberry)polysaccharides were extracted from L.barbarum wine,the components were determined,and its effect on alcoholic liver disease was studied.Sixty male C57BL/6 mice were randomly divided into control group,model group,control+L.barbarum polysaccharides(LBP)group and model+LBP group.The contents of alanine aminotransfease(ALT)aspartate aminotransferase(AST)and lipopolysaccharide(LPS)were determined,and the changes of inflammatory factors in serum and liver were detected by enzyme-linded immunosorbent assay(ELISA).The results showed that the concentration of polysaccharides extracted from L.barbarum wine was 1.2 g/L,in which the mineral elements Ca,P,Mg,K and Na were 0.09%,0.69%,0.28%,3.92%and 0.51%,respectively.The contents of aspartic acid(ASP)and glutamic acid(GLU)in 17 amino acids were higher,which were 7.25%and 4.96%,respectively.Compared with the model group,the contents of ALT,AST and LPS in the model+LBP group were significantly lower(P<0.05),and the contents of inflammatory cytokines IL-1β,IL-6 and TNF-αwere significantly lower than those in the model group(P<0.05).The results showed that polysaccharides from L.barbarum wine could significantly alleviate alcoholic liver injury.

关 键 词：枸杞酒多糖 提取 成分测定 酒精肝 

分 类 号：Q815[生物学—生物工程]