黄瓜中毒死蜱残留基体标准物质的研制  

Preparation of matrix reference material for chlorpyrifos residues in cucumber

作　　者：成长玉 钟慈平 黄丽娟 黄瑛 余晓琴 CHENG Chang-Yu;ZHONG Ci-Ping;HUAGN Li-Juan;HUAGN Ying;YU Xiao-Qin(Sichuan Institute for Food and Drug Control,Chengdu 611731,China)

机构地区：[1]四川省食品药品检验检测院,成都611731

出　　处：《食品安全质量检测学报》2020年第18期6356-6361,共6页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2017YFC1601300)。

摘　　要：目的建立黄瓜中毒死蜱残留基体标准物质的研制方法。方法选择符合标准物质研制要求的新鲜黄瓜为研制对象,经过预冻干、添加、混匀、二次冻干、过筛、包装及辐照灭菌,采用气相色谱-串联质谱法测定,同位素内标法定量,经均匀性和稳定性检验后,采取多家实验室合作定值的方式进行定值。结果通过11家独立实验室合作定值及不确定度评估,毒死蜱的特性值为(0.403±0.033)mg/kg(k=2)。结论获得的样品均匀性和稳定性良好,适用于黄瓜中毒死蜱检测过程的方法研制和质量控制。Objective To establish a method for the preparation of chlorpyrifos residues in cucumber.Methods Fresh cucumber which meets the requirements of reference material development was selected as the research object,after pre freeze-drying,adding,mixing,secondary freeze-drying,screening,packaging and irradiation sterilization,the cucumber was determined by gas chromatography tandem mass spectrometry(GC-MS/MS)and quantified by isotope internal standard method.After homogeneity and stability test,the value was determined by cooperation of several laboratories.Results The property value and expanded uncertainty of chlorpyrifos was(0.403±0.033)mg/kg(k=2),certified by 11 independent laboratories.Conclusion The uniformity and stability of the samples are good,which is suitable for the development of the method and quality control of chlorpyrifos poisoning in cucumber.

关 键 词：标准物质 黄瓜 毒死蜱 定值 不确定度 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]