高效液相色谱法测定饲料中喹烯酮  

Determination of quincetone in feed by high performance liquid chromatography

作　　者：侯林丛 陆静 梁辰 刘丹丹 商方方[4] 李俊[4] HOU Lin-Cong;LU Jing;LIANG Chen;LIU Dan-Dan;SHANG Fang-Fang;LI Jun(Henan Hairuizheng Testing Technology Co.,Ltd.,Zhengzhou 450001,China;School of Bioengineering,Henan University of Technology,Zhengzhou 450001,China;Shijiazhuang Veterinary Biological Products Supply Station,Shijiazhuang 050040,China;Feed Research Institute of Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]河南海瑞正检测技术有限公司,郑州450001 [2]河南工业大学生物工程学院,郑州450001 [3]石家庄市兽用生物制品供应站,石家庄050040 [4]中国农业科学院饲料研究所,北京100081

出　　处：《食品安全质量检测学报》2020年第18期6672-6677,共6页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2018YFC1603401-02)。

摘　　要：目的建立高效液相色谱法测定饲料中喹烯酮的分析方法。方法样品经体积分数80%甲醇溶液作为提取液,超声提取10 min,重复提取2次,用HLB固相萃取柱进行净化,以甲醇:水(60:40, V:V)作为流动相进行分离,经0.45μm滤膜过滤后用高效液相色谱仪进行测定。结果样品溶液浓度在0.10~10.0μg/mL范围内,其吸光度和浓度呈良好的线性关系r^2>0.9998,方法的检出限为0.1mg/kg,平均回收率为87.5%~96.4%,相对标准偏差为1.39%~4.03%。结论该方法操作简单、准确度高、重复性好,适合大批量饲料样品中喹烯酮的检测。Objective To establish a method for the determination of quincetone in feedstuffs by high performance liquid chromatograph(HPLC).Methods The sample was extracted with 80%methanol solution,ultrasonically extracted for 10 min,and extracted twice,purified with an HLB solid phase extraction column,and separated with methanol:water(60:40,V:V)as the mobile phase.After being filtered by a 0.45μm filter membrane,it was measured by HPLC.Results When the concentration of sample solution was in the range of 0.10-10.0μg/mL,the linear relationship was good,with r^2>0.9998,and the detection limit was 0.1 mg/kg.The average recovery was 87.5%-96.4%,and the relative standard deviation(RSD)was 1.39%-4.03%.Conclusion This method is simple,accurate and repeatable,which is suitable for the detection of quincetone in large quantities of feed samples.

关 键 词：喹烯酮 饲料 高效液相色谱法 

分 类 号：S816.17[农业科学—饲料科学]