抚顺辽五味子二维相关荧光光谱鉴别方法研究  

Identification of Schisandra chinensis Liaoning Fushun by two-dimensional correlation fluorescence spetroscopy

作　　者：陈培云 李红莲[2] 赵志磊[2] 徐唐芸 CHEN Pei-Yun;LI Hong-Lian;ZHAO Zhi-Lei;XU Tang-Yun(College of Biological and Environmental Science,Zhejiang Wanli University,Ningbo 315000,China;College of Quality and Technial Supervision,Hebei University,Baoding 071002,China)

机构地区：[1]浙江万里学院生物与环境学院,宁波315000 [2]河北大学质量技术监督学院,保定071002

出　　处：《食品安全质量检测学报》2020年第18期6702-6706,共5页Journal of Food Safety and Quality

基　　金：2020年浙江万里学院大学生创新训练项目。

摘　　要：目的建立对抚顺辽产地的五味子快速识别的方法。方法采用荧光分光光度计检测抚顺辽产地的五味子果实样品的三维荧光光谱,结合不同激发波长和发射波长彼此间波长间隔扰动下的二维相关荧光光谱技术。结果本方法分析抚顺辽产地与其他产地的五味子的二维同步相关光谱的形状、自相关峰及交叉峰的出峰个数、位置、正负和强度等都存在明显不同。结论抚顺辽产地与其他产地的二维相关荧光光谱差别十分明显和直观,可以作为抚顺辽原产地鉴别的一种有效方法。Objective To establish a rapid identification method of Schisandra chinensis Liaoning Fushun.Methods Three-dimensional fluorescence spectroscopy of Schisandra chinensis Liaoning Fushun was obtained by fluorescence spectrometry and combined with two-dimensional correlation fluorescence spectroscopy under disturbance of the gap between different excitation and emission wavelength.Results The shape,number,position,positive and negative,and intensity between the auto-peaks and cross-peaks of two-dimensional synchronous fluorescence spectra of Schisandra chinensis from Fushun and other places were obviously different.Conclusion The difference of two-dimensional correlation fluorescence spectrum between Fushun and other places is very obvious and intuitive,which can be used as an effective method to identify the origin of Fushun in Liaoning province.

关 键 词：抚顺辽五味子 三维荧光光谱法 同步荧光光谱法 二维相关光谱法 

分 类 号：R282.5[医药卫生—中药学] O657.3[医药卫生—中医学]