维拉帕米对耐药细菌的体外增敏性效果  

Effect of Verapamil on Reducing Drug Sensitivity of 2 Drug-Resistant Xylose Staphylococcus

作　　者：张忠斌 丁文雅 张金娜[2] Zhang Zhongbin(Guangxi University of Traditional Chinese Medicine,Nanning Guangxi 530200)

机构地区：[1]广西中医药大学,广西南宁530200 [2]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《黑龙江医药》2020年第5期982-984,共3页Heilongjiang Medicine journal

基　　金：中国博士后科学基金(编号:2019M661246);黑龙江省普通本科高等学校青年创新人才培养(编号:UNPYSCT-2016133)

摘　　要：目的:考察维拉帕米对耐泰乐菌素木糖葡萄球菌、耐阿奇霉素木糖葡萄球菌的药物增敏性。方法:利用阿奇霉素和泰乐菌素两种大环内酯类抗生素诱导木糖葡萄球菌耐药菌株。通过倍比稀释法测定维拉帕米的剂量与加入时间对耐药菌株最小抑菌浓度(MIC值)影响情况。结果:维拉帕米对木糖葡萄球菌的MIC值无影响,而0.5mg/mL的维拉帕米能显著降低2种耐药菌株MIC值,提前2h加入0.25mg/mL维拉帕米也可以降低2种耐药菌株MIC值。结论:维拉帕米可以增强耐药木糖葡萄球菌对药物的敏感性,且这种影响作用与药物加入的时间、剂量具有一定相关性。Objective:To observe the effect of verapamil on reducing bacterial resistance of 2 drug-resistant xylose staphylococcus.Methods:Two strains of xylose staphylococcus were respectively induced in vitro by tylosin and azithromycin.The minimal inhibitory concentration(MIC)were tested by dilution method with different concentrations and administration timing of verapamil.Results:The MIC of drug-resistant xylose staphylococcus were significantly decreased after 0.5mg/mL verapamil added.If The drug-resistant xylose staphylococcus were cultivated 2h with 0.25mg/mL verapamil before tylosin and azithromycin added,the MIC values of drugs against xylose staphylococcus reduced significantly.Conclusions:Verapamil can enhanced the drug sensitivity of drug-resistant xylose staphylococcus,and this effect is correlated with the time and dose of drug.

关 键 词：维拉帕米 木糖葡萄球菌 MIC值 

分 类 号：R978.1[医药卫生—药品]