PTPRO在溃疡性结肠炎组织中的表达及意义  

Expression and significance of PTPRO in ulcerative colitis

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作  者:葛倩文 吴尚农[1] 王宏刚[1] 沈鹏[1] GE Qianwen;WU Shangnon;WANG Honggang;SHEN Peng(Department of Gastroenterology,Huai′an First People′s Hospital,Huai′an 223001,China)

机构地区:[1]淮安市第一人民医院消化科,223001

出  处:《国际消化病杂志》2020年第5期309-313,共5页International Journal of Digestive Diseases

摘  要:目的探究受体型蛋白酪氨酸磷酸酶O(PTPRO)在溃疡性结肠炎(UC)组织中的表达及意义。方法前瞻性选取2014年4月至2017年8月在淮安市第一人民医院就诊的95例UC患者作为研究对象,设为UC组;选取本院同期行肠镜检查并切除肠内息肉的46例患者作为对照组。采用蛋白质免疫印迹法检测UC组织中PTPRO蛋白表达量,并分析其与UC镜下活动度及预后的关系。结果UC组PTPRO蛋白表达量高于对照组,差异有统计学意义(P<0.05)。镜下缓解期UC患者PTPRO蛋白表达量低于镜下活动期UC患者,差异有统计学意义(P<0.05)。PTPRO蛋白表达量评价UC镜下活动度的受试者工作特征(ROC)曲线下面积(AUC)、敏感度及特异度分别为0.899、85.71%和78.33%。UC患者中预后良好组PTPRO蛋白表达量低于预后不良组,差异有统计学意义(P<0.05)。PTPRO蛋白表达量评价UC患者预后的ROC AUC、敏感度及特异度分别为0.957、93.75%和87.30%。Logistic回归方法分析结果显示,PTPRO、UC内镜下严重程度指数(UCEIS)及抗中性粒细胞胞浆抗体(ANCA)与UC患者预后关系密切。结论PTPRO在UC组织中高表达,检测其表达水平有助于评估UC患者镜下活动度及预后情况。Objective This paper aims to investigate the expression and significance of protein tyrosine phosphatase receptor-type O(PTPRO)in ulcerative colitis(UC).Methods A prospective study of 95 patients with UC who were admitted in Huai′an First People′s Hospital from April 2014 to August 2017 was included and set as the UC group while 46 patients who underwent colonoscopy and resection of intestinal polyps were selected as the control group.Western blotting was used to detect the expression of PTPRO protein in UC tissues,and its relationship with activity under microscope of UC and prognosis was analyzed.Results The expression of PTPRO protein in the UC group was higher than that in the control group,with a statistically significant difference(P<0.05).The expression of PTPRO protein in UC patients in remission period was lower than that in UC patients in active period(P<0.05).The area under the receiver operating characteristic(ROC)curve area(AUC),sensitivity and specificity of PTPRO protein expression to evaluate the mobility under UC microscope were 0.899,85.71%and 78.33%respectively.The expression of PTPRO protein in UC patients with good prognosis was lower than that in UC patients with poor prognosis(P<0.05).The ROC AUC,sensitivity and specificity of PTPRO protein expression in evaluating the prognosis of UC patients were 0.957,93.75%and 87.30%,respectively.Logistic regression analysis showed that PTPRO,UCEIS score and ANCA were closely related to the prognosis of patients with UC.Conclusion PTPRO is highly expressed in UC tissues,and its expression is helpful for the evaluation of microscopic activity and prognosis in patients with UC.

关 键 词:溃疡性结肠炎 受体型蛋白酪氨酸磷酸酶O 镜下活动度 预后 

分 类 号:R73[医药卫生—肿瘤]

 

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