IL-10多态性对牙周炎患者牙周微生态的影响  

Effect of IL-10 gene polymorphism on periodontal microecology in patients with periodontitis

作　　者：陈晓华 徐茂林 CHEN Xiao-hua;XU Mao-lin(Department of Stomatology,Zibo First Hospital.Zibo 255200,Shandong Province,China;Department of Otorhinolaryngology,Zibo First Hospital.Zibo 255200,Shandong Province,China)

机构地区：[1]淄博市第一医院口腔科,山东淄博255200 [2]淄博市第一医院耳鼻咽喉科,山东淄博255200

出　　处：《上海口腔医学》2020年第5期533-538,共6页Shanghai Journal of Stomatology

摘　　要：目的:探讨IL-10多态性对牙周炎患者牙周微生态的影响。方法:选取2018年6月—2019年3月淄博市第一医院收治的牙周炎患者作为实验组(n=79),按照牙周炎类型分为中度牙周炎(n=47)与重度牙周炎(n=32)。另选择同期来院体检的牙周健康人作为对照组(n=50)。采用实时荧光定量PCR技术检测所有研究对象龈下牙菌斑中细菌定植水平;抽取静脉血,通过PCR技术检测IL-10中的-1082、-819、-592位点上的基因型及等位基因频率。通过多因素分析,探讨IL-10不同基因型与龈下牙菌斑中细菌定植水平的关系。采用SPSS 22.0软件包对数据进行统计学分析。结果:中度牙周炎患者伴放线放线杆菌(Actinomycetes actinomycetes,A.a)定植量显著低于重度牙周炎患者(P<0.05);中度牙周炎与重度牙周炎患者牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)及A.a定植量均显著高于对照组(P<0.05)。中度牙周炎患者中,-819位点的CC基因型比例显著低于对照组,TT基因型比例显著高于对照组(P<0.05);-819位点CC、TC、TT基因型比例与重度牙周炎患者相比无显著差异(P>0.05);-592位点的CC基因型比例显著低于对照组,AA基因型比例显著高于对照组(P<0.05)。中度牙周炎中,-592位点的AA基因型患者A.a定植量显著高于本组AC基因型患者(P<0.05);-819位点的TT基因型患者A.a定植量显著高于本组TC基因型患者(P<0.05)。多因素分析显示,中度牙周炎中IL-10-592-AA基因型、-819-TT基因型与A.a定植水平密切相关(P<0.05)。结论:IL-10基因多态性是中度牙周炎患者龈下微生态环境的影响因素,其中,IL-10-592-AA基因型、-819-TT基因型与A.a定植水平相关性最明显。PURPOSE:To investigate the effect of IL-10 gene polymorphism on periodontal microecology in patients with periodontitis.METHODS:Seventy-nine patients with periodontitis(experimental group)and 50 healthy individuals(control group)visiting Zibo First Hospital from June 2018 to March 2019 were enrolled.The experimental group was further divided into two groups according to the severity of periodontitis,namely moderate periodontitis(n=47)and severe periodontitis(n=32).Real-time quantitative PCR was used to detect the level of bacterial colonization in the subgingival plaque of all subjects.The genotype and allele frequencies at-1082,-819,and-592 loci in IL-10 were detected by PCR.Multivariate analysis was used to explore the relationship between IL-10 genotypes and bacterial colonization level in subgingival plaque.The data were processed by SPSS 2.0 software package.RESULTS:Actinomycetes actinomycetes(A.a)colonization in patients with moderate periodontitis was significantly lower than that of patients with severe periodontitis(P<0.05).The amount of Porphyromonas gingivalis(P.g)and A.a in patients with moderate periodontitis and severe periodontitis was significantly higher than that in the control group(P<0.05).The proportion of CC genotypes in the-819 locus of patients with moderate periodontitis was significantly lower than that of the control group,and the proportion of TT genotype was significantly higher than that of the control group(P<0.05).The proportion of CC,TC and TT genotypes showed no significant difference between patients with moderate periodontitis and those with severe periodontitis(P>0.05).The proportion of CC genotypes in the-592 locus of patients with moderate periodontitis was significantly lower than that of the control group,and the proportion of AA genotype was significantly higher than that of the control group(P<0.05).In moderate periodontitis,A.a colonization amount at-592 gene locus of AA genotype was significantly higher than that of AC genotype(P<0.05).In moderate periodontitis,A.a col

关 键 词：IL-10基因多态性 牙周炎 牙周微生态 

分 类 号：R781.4[医药卫生—口腔医学]