黄芩素通过上调miR-7-5p影响脂多糖诱导的肾小球上皮细胞氧化应激及凋亡  

Scutellarin affects LPS-induced oxidative stress and apoptosis of glo⁃merular epithelial cells by up-regulating miR-7-5p

作　　者：周玉娣 蒋为 周萍 ZHOU Yu-di;JIANG Wei;ZHOU Ping(Department of Nephrology,Zhuji People's Hospital,Zhuji 311800,China.)

机构地区：[1]诸暨市人民医院肾内科,浙江诸暨311800

出　　处：《中国病理生理杂志》2020年第10期1860-1866,共7页Chinese Journal of Pathophysiology

基　　金：浙江省医药卫生科技计划项目。

摘　　要：目的:探讨黄芩素(SCU)对脂多糖(LPS)诱导的人肾小球上皮细胞氧化应激和凋亡的影响及其机制。方法:体外培养人肾小球上皮细胞,LPS(1.0 mg/L)处理建立细胞损伤模型,分为正常对照(NC)组、LPS组、NC+SCU组、LPS+SCU组、LPS+miR-NC组、LPS+微小RNA-7-5p(miR-7-5p)组、LPS+SCU+anti-miR-NC组和LPS+SCU+anti-miR-7-5p组。CCK-8法检测细胞活力;流式细胞术检测细胞凋亡;试剂盒测定细胞内丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,以及细胞培养上清液中乳酸脱氢酶(LDH)活性;RT-qPCR检测miR-7-5p的表达水平。结果:与NC组比较,LPS组细胞活力、miR-7-5p表达和SOD活性显著降低,细胞凋亡率、MDA含量和LDH活性显著升高(P<0.05);与LPS组比较,LPS+SCU组细胞活力、miR-7-5p表达和SOD活性显著升高,细胞凋亡率、MDA含量和LDH活性显著降低(P<0.05);与LPS+miR-NC组比较,LPS+miR-7-5p组细胞活力和SOD活性显著升高,细胞凋亡率、MDA含量和LDH活性显著降低(P<0.05);与LPS+SCU+anti-miR-NC组比较,LPS+SCU+anti-miR-7-5p组细胞活力和SOD活性显著降低,细胞凋亡率、MDA含量和LDH活性显著升高(P<0.05)。结论:黄芩素通过上调miR-7-5p表达抑制LPS诱导的肾小球上皮细胞氧化应激损伤和凋亡。AIM:To investigate the effect of scutellarin(SCU)on oxidative stress and apoptosis induced by li⁃popolysaccharide(LPS)in human glomerular epithelial cells and its mechanism.METHODS:Human glomerular epithe⁃lial cells were cultured in vitro,and were treated with LPS(1.0 mg/L)to establish a cell injury model.The cells were di⁃vided into normal control(NC)group,LPS group,NC+SCU group,LPS+SCU group,LPS+miR-NC group,LPS+mi⁃croRNA-7-5p(miR-7-5p)group,LPS+SCU+anti-miR-NC group and LPS+SCU+anti-miR-7-5p group.Cell viability was detected by CCK-8 assay.Apoptosis was detected by flow cytometry.The intracellular malondialdehyde(MDA)content and superoxide dismutase(SOD)activity,and lactate dehydrogenase(LDH)activity in the cell culture supernatant were determined by kit.RT-qPCR was used to detect the expression level of miR-7-5p.RESULTS:Compared with NC group,the cell viability,miR-7-5p expression and SOD activity in LPS group were significantly reduced,and the apoptotic rate,MDA content and LDH activity were significantly increased(P<0.05).Compared with LPS group,the cell viability,miR-7-5p expression and SOD activity in LPS+SCU group were significantly increased,and the apoptotic rate,MDA content and LDH activity were significantly reduced(P<0.05).Compared with LPS+miR-NC group,the cell viability and SOD activity in LPS+miR-7-5p group were significantly increased,and the apoptotic rate,MDA content and LDH activity were significantly reduced(P<0.05).Compared with LPS+SCU+anti-miR-NC group,the cell viability and SOD activity in LPS+SCU+anti-miR-7-5p group were significantly reduced,and the apoptotic rate,MDA content and LDH activity were significantly increased(P<0.05).CONCLUSION:Scutellarin inhibits LPS-induced oxidative stress damage and apopto⁃sis in glomerular epithelial cells via up-regulating miR-7-5p expression.

关 键 词：黄芩素 微小RNA-7-5p 脂多糖 肾小球上皮细胞 氧化应激 细胞凋亡 

分 类 号：R363.2[医药卫生—病理学] R329.25[医药卫生—基础医学]