卵清蛋白(OVA)致敏联合烟熏法建立咳嗽变异型哮喘豚鼠模型  

Establishment of a cough variant asthma guinea pig model induced by cigarette smoke exposure and OVA sensitization

作　　者：吕天宜 李得民[1] 杨道文[1] 程思益 LYU Tianyi;LI Demin;YANG Daowen;CHENG Siyi(Pulmonary Disease Department,China-Japan Friendship Hospital,National Respiratory Disease Clinical Research Center,Beijing 100029,China)

机构地区：[1]中日友好医院中医肺病科中日友好医院呼吸中心国家呼吸疾病临床研究中心,北京100029

出　　处：《中国比较医学杂志》2020年第10期1-7,共7页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金资助项目(81704045)。

摘　　要：目的尝试用卵清蛋白联合烟熏建立相似度更高的豚鼠CVA模型。方法选取SPF级雌雄各半豚鼠24只,随机分为空白对照组、OVA致敏组与OVA致敏+烟熏组,共3组,每组8只豚鼠。OVA致敏组以卵清蛋白致敏法造模,OVA致敏+烟熏组以卵清蛋白致敏联合烟熏法造模,余方法一致。检测3组受试豚鼠肺顺应性(Cydn)、乙酰甲胆碱激发后气道阻力(RI)、辣椒素激发后咳嗽次数与首次咳嗽时间、肺组织病理及Ashcroft分级表评分指标变化,以评估模型。结果相较于空白对照组,OVA致敏组与OVA致敏+烟熏组Cydn明显下降、RI明显上升、10 min咳嗽次数明显增加、首次咳嗽时间明显提前、肺组织病理变化明显、Ashcroft分级表评分明显上升,以上表现均符合咳嗽变异型哮喘特征。同时,相较于OVA致敏组,OVA致敏+烟熏组在咳嗽症状及RI变化上有更好表现,更符合咳嗽变异型哮喘特征,这可能与降低OVA致敏浓度避免免疫反应过度、烟熏增加咳嗽敏感性密切相关。结论用OVA致敏联合烟熏的方法能建立相似度跟高的咳嗽变异型哮喘豚鼠模型。Objective To establish a cough variant asthma(CVA)guinea pig model induced by cigarette smoke exposure and OVA sensitization.Methods Twenty-four specific pathogen-free guinea pigs were randomLy assigned to three experimental groups:control(n=8),OVA(n=8),and CS(cigarette smoke)+OVA(n=8).Guinea pigs in the OVA group were sensitized with ovalbumin(OVA)and those in the CS+OVA group were sensitized with ovalbumin and exposed to cigarette smoke.At the end of experiment,airway resistance(RI)challenged by four doses of methacholine(0.2 mL,0.4 mL,0.6 mL,0.8 mg/mL)and lung compliance(Cydn)were measured to determine airway hyper-responsiveness(AHR),and cough latency and the frequency of coughing challenged by capsaicin within 10 minutes were analyzed to evaluate symptoms.Hematoxylin and eosin staining and the Ashcroft score were used to observe the state of lung injury.Results Compared with the control group,RI,Ashcroft score,and the frequency of coughing in OVA and OVA+CS groups were increased significantly,and Cydn and cough latency were decreased significantly.Compared with the OVA group,the OVA+CS group had better performance in terms of cough symptoms and RI,which was more similar to the characteristics of cough variant asthma.This may be closely related to appropriate reduction of the OVA dose.Conclusions The CS+OVA guinea pig model is more consistent with the clinical features of cough variant asthma and can be used as an animal model of cough variant asthma.

关 键 词：呼吸系病 CVA豚鼠模型 动物实验 卵清蛋白 烟熏 

分 类 号：R-33[医药卫生]