IVC换笼标准操作程序与小鼠病毒病原的传播风险  

Individual ventilated cage changing standard operating procedure and viral pathogen control in laboratory mice

作　　者：佟巍[1] 郭智 刘子洋 潘思丹 高虹[1] 向志光[1] TONG Wei;GUO Zhi;LIU Ziyang;PAN Sidan;GAO Hong;XIANG Zhiguang(Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences,Comparative Medical Center,Peking Union Medical College,Beijing 100021,China)

机构地区：[1]中国医学科学院医学实验动物研究所北京协和医学院比较医学中心,北京100021

出　　处：《中国比较医学杂志》2020年第10期107-109,共3页Chinese Journal of Comparative Medicine

基　　金：十三五国家重大专项(2017ZX10304402-001-009);国家重点研发计划(2016YFD0501003)。

摘　　要：目的研究独立通风笼具换笼标准操作程序与实验动物负压屏障设施内使用IVC(individual ventilated cage,IVC)设备饲养小鼠的病毒感染风险。方法选择小鼠易感的小鼠肝炎病毒(MHV)A59株感染6周龄BALB/c雄性小鼠,按照IVC换笼标准操作程序进行换笼操作;使用IFA和ELISA血清学方法以及针对MHV的核酸检测方法测定同一笼架具相邻笼盒、同一IVC系统不同笼架具的不同位置、以及IVC系统外负压屏障设施内哨兵动物的MHV感染情况。结果接种病毒小鼠在感染6 d后出现临床症状,粪便中可检出MHV病原;动物感染3周后可检出MHV抗体阳性;其他监测组在5周实验终点均无MHV阳性检出。结论IVC换笼标准操作程序可指导IVC换笼操作,用以控制病毒类病原对小鼠的感染风险。Objective To evaluate the effect of the individual ventilated cage(IVC)changing standard operating procedure(SOP)for viral pathogen transmission in laboratory mice.Methods Six-week-old male BALB/c mice were infected with mouse hepatitis virus(MHV,A59 strain).The infection status of these infected mice and sentinel mice in close cages on the same shelf,in cages on a different shelf but in the same IVC system,and outside the IVC system but in the same facility were monitored.Serological conversion of MHV antibodies was detected using immunofluorescent antibody reaction and enzyme-linked immunosorbent assay.MHV in mouse feces was detected using reverse transcription polymerase chain reaction.Results MHV nuclear acid was detected 6 d after infection in the artificial infected mice,and serological conversion was detected 3 weeks postinfection.At 5 weeks postinfection,there was no positive detection in sentinel mice.Conclusions The IVC-changing SOP is useful for pathogen transmission management in laboratory mice.

关 键 词：IVC 换笼标准操作程序 MHV 小鼠 

分 类 号：R-33[医药卫生]