基于粒子图像测速技术的急性高眼压兔眼在体实验研究  

In vivo experimental study of acute intraocular hypertension in rabbit based on particle image velocimetry technique

作　　者：李琦 王文佳 龙晓雪 杨宏宇 宋红芳 LI Qi;WANG Wenjia;LONG Xiaoxue;YANG Hongyu;SONG Hongfang(Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application,Beijing 100069,P.R.China;School of Biomedical Engineering,Capital Medical University,Beijing 100069,P.R.China;Beijing Tiantan Hospital,Capital Medical University,Beijing 100070,P.R.China)

机构地区：[1]临床生物力学应用基础研究北京市重点实验室,北京100069 [2]首都医科大学生物医学工程学院,北京100069 [3]首都医科大学附属北京天坛医院,北京100070

出　　处：《生物医学工程学杂志》2020年第5期818-824,共7页Journal of Biomedical Engineering

基　　金：国家自然科学基金(10802053);北京市自然科学基金(7152022)。

摘　　要：目前对于在体眼内房水流动的实验研究较少,相关技术也不成熟。本研究旨在利用粒子图像测速(PIV)技术,探索急性高眼压下兔眼前房这种低速流场的在体测量实验方法和关键技术。研究内容包括急性高眼压兔眼造模、在体测试眼球准备和PIV系统搭建三个部分。重点探究用于兔眼PIV实验的荧光粒子最佳注射方案,以及图像折射和呼吸影响带来的图像采集误差修正方案。研究结果显示,采用15μL荧光粒子溶液从虹膜下方缓慢注入兔眼前房后回笼等待13 h,使粒子借助房水循环均匀分布在前房,即可进行在体PIV实验测量;实验时,需使用带超声耦合胶的方形水槽盖在眼上进行摄像;对测量结果进行后处理计算前,可采用最大互信息算法对数据进行校正。最终研究结果表明,在用PIV技术对急性高眼压兔眼进行在体前房房水流场测试时,选定合适的荧光粒子注射方案及图像采集修正方案对于得到良好的实验效果至关重要。At present,there are few in vivo experimental studies on anterior chamber flow field,and the relevant technologies are not mature.This study explores the experimental method and key techniques of particle image velocimetry(PIV)for the in vivo measurement of anterior chamber flow field with slow flow velocity in the rabbit with acute intraocular hypertension.The experimental process can be divided into three parts:model construction of rabbit eye with acute intraocular hypertension,in vivo eyeball preparation,and PIV setup.The following key techniques were mainly investigated:the optimal injection strategy of fluorescent particles and the correction strategy for image acquisition errors caused by the effects of image refraction and respiration.The results showed that the best injection method was that 15μL of fluorescent particles solution was slowly injected into the anterior chamber through the lower part of iris and then the rabbit was released and waited for 13 h.In this way particles were completely distributed in the anterior chamber with the help of the aqueous humor circulation,and then in vivo PIV experiment could be performed.The eyeball should be covered with a square flume filled with ultrasonic coupling gel for the sake of imaging during the experiment.The Maximal Information Coefficient algorithm could be applied to correct the measured results before post-processing calculation.The results indicated that feasible injection strategy of fluorescent particles and the correction strategy for image acquisition are critical to obtain nice experiment effects for the in vivo PIV measurement of anterior chamber flow field in the rabbit with acute intraocular hypertension.

关 键 词：青光眼 急性高眼压 房水 流体力学 粒子图像测速技术 

分 类 号：R775[医药卫生—眼科]