还原石墨烯聚苯胺Langmuir-Blodgett膜的胆固醇生物传感器的研究  

Reduced Graphene and Polyaniline LB Films as Electrode for Detection of Cholesterol

作　　者：芦菲[1] 戴俊琦 Lu Fei;Dai Junqi(College of Chemical&Pharmaceutical Engineering,Jilin Institute of Chemical Technology,Jilin 132022,China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022

出　　处：《广东化工》2020年第18期12-13,2,共3页Guangdong Chemical Industry

基　　金：2017年吉林市青年科技工作者创新创业大赛(201750253)。

摘　　要：本文通过用Langmuir-Blodgett(LB)膜技术制得了还原石墨烯/酶/聚苯胺LB膜电极,并采用紫外—可见吸收光谱法、红外光谱法进行表征。并且研究了胆固醇在制备的修饰电极上的电化学行为。结果表明:采用LB膜制备还原石墨烯,聚苯胺支持的电化学传感器,检测胆固醇的线性范围为5~100μg/mL,检出限为5μg/mL。此外,对葡萄糖、抗坏血酸、乳酸和尿酸的敏感性较低。研究成果在胆固醇快速检测中具有潜在的应用价。In this paper,the reduced graphene/enzyme/polyaniline LB film electrode was prepared by using LB technology.The electrode was characterized via infrared spectrometry and uv-visible absorption spectrum.At the same time the behaviour of reduced graphene/enzyme/polyaniline LB film electrode were studied.The cholesterol sensor of LB films had a linear detection range between 5and 100 mg/dL.In addition,the results confirmed low cross-sensitivities toward glucose,ascorbic acid,acetaminophen and uric acid.

关 键 词：电化学生物传感器 Langmuir-Blodgett薄膜技术 胆固醇检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]