野生和栽培蓝莓果中总多酚含量的比较  

Comparison of the total polyphenol content in wild and cultivated blueberry

作　　者：赵婧 李成 杨长青 金贞姬 ZHAO Jing;LI Cheng;YANG Changqing;JIN Zhenji(School of Basic Medicine and Clinic Pharmacy,China Pharmaceutical University,Nanjing 211198,Jiangsu,China;Departmentof Pharmacy,Affiliated Hospital of Yanbian University,Yanji 133000,Jilin,China;Nanjing BRT-Biomed Co.,Ltd.,Nanjing 211100,Jiangsu,China)

机构地区：[1]中国药科大学基础医学与临床药学学院,江苏南京211198 [2]延边大学附属医院药学部,吉林延吉133000 [3]南京珼瑞斯生物医药科技有限公司,江苏南京211100

出　　处：《延边大学医学学报》2020年第1期10-13,共4页Journal of Medical Science Yanbian University

基　　金：国家自然科学基金资助项目(No.81660619).

摘　　要：[目的]比较野生和栽培蓝莓果中的总多酚含量.[方法]采用Folin-Ciocalteu比色法比较野生和栽培蓝莓果中的总多酚含量.分别利用超声提取法和酸提法提取蓝莓果中的可萃取多酚(EPP)和不可萃取多酚(NEPP),以没食子酸为标准品,绘制浓度-吸光度标准曲线,根据线性回归方程计算总多酚含量.[结果]没食子酸在0~5 mg/L质量浓度范围内与吸光度呈良好的线性关系(Y=0.0784 X+0.0069,r=0.9983),最低检测质量浓度为0.5 mg/L,野生蓝莓果中总多酚含量为(53.01±1.27)mg/g,栽培蓝莓果中总多酚含量为(17.56±0.28)mg/g.[结论]野生蓝莓果中总多酚含量高于栽培蓝莓果.野生蓝莓更适合做保健品原料.OBJECTIVE To compare the total polyphenol content in wild and cultivated blueberry.METHODS Folin-Ciocalteu colorimetric method was used to compare the content of total polyphenols in wild and cultivated blueberry.Extractable polyphenols(EPP)and non-extractable polyphenols(NEPP)in blueberries were extracted by ultrasonic extraction and acid extraction method respectively.Gallic acid was used as the standard substance to draw the concentration-absorbance standard curve,and the total polyphenol content was determined by linear regression equation.RESULTS Gallic acid showed a good linear relationship with absorbance in the range of 0-5mg/L mass concentration(Y=0.0784 X+0.0069,r=0.9983),and the minimum detected mass concentration was 0.5mg/L.The total polyphenol content of wild blueberry was(53.01±1.27)mg/g,and that of cultivated blueberry was(17.56±0.28)mg/g.CONCLUSION The content of total polyphenols in wild blueberry is higher than that in cultivated blueberry.Wild blueberry is more suitable to be used as raw materials of healthcare products.

关 键 词：蓝莓果 总多酚 Folin-Ciocalteu比色法 含量比较 

分 类 号：R284[医药卫生—中药学]