酶法工艺头孢丙烯中酶蛋白残留的检测  

Determination of Residual Protein in Cefprozil by Enzymatic Synthesis

作　　者：李庆 关晴 刁富城 Li Qing;Guan Qing;Diao Fucheng(Guangzhou Baiyunshan Pharmaceutical Holdings Co.,Ltd.,Baiyunshan Chemical Pharmaceutical Factory,Guangzhou 510515,China)

机构地区：[1]广州白云山医药集团股份有限公司白云山化学制药厂,广东广州510515

出　　处：《广东化工》2020年第20期100-102,共3页Guangdong Chemical Industry

基　　金：广州市珠江科技新星专项资助(201710010121)。

摘　　要：目的:建立一种酶法工艺制备的头孢丙烯中酶蛋白残留的新检测方法。方法:采用考马斯亮蓝法,配制标准蛋白线性溶液建立标准曲线,取考马斯亮蓝G-250染液加入到10%碳酸氢钠溶液溶解的头孢丙烯供试品溶液中,混匀反应10分钟后使用紫外分光光度计测定吸光度,采用标准曲线法计算得到供试品溶液中的蛋白浓度,并计算出供试品所含酶蛋白残留含量。结果:酶蛋白在25~1000μg/mL(相当于供试品0.05%~2.0%)浓度范围内与吸光度呈良好线性关系(r=0.9976);精密度RSD(n=12)为4.5%;平均回收率(n=12)为98.9%。结论:本方法简单便捷、重复性好、快速准确,适用于酶法工艺制备的头孢丙烯中酶蛋白残留的含量测定。Objective:To establish a new method for the determination of residual protein in cefprozil prepared by enzymatic synthesis.Methods:According to Bradford method,prepare the standard protein linear solution to establish the standard curve.Bradford G-250 dye solution was added into the cefprozil sample dissolved in 10%sodium bicarbonate solution.The absorbance was determined by UV spectrophotometer after 10 min mixing reaction.The residual protein concentration in sample solution was calculated by standard curve,then the residual enzyme protein was calculated out.Results:there was a good linear relationship(r=0.9976)between enzyme protein and UV absorbance in the concentration range of 25~1000μg/mL(equivalent to 0.05%~2.0%of sample);the RSD(n=12)of the precision was 4.5%;the average recovery(n=12)was 98.9%.Conclusion:The method is convenient,reproducible,rapid and accurate,which is suitable for the determination of residual enzyme protein in cefprozil prepared by enzymatic synthesis.

关 键 词：头孢丙烯 酶法 蛋白残留 含量测定 

分 类 号：R927[医药卫生—药学]