川楝素通过下调环状RNA circDLST对胃癌细胞BGC-823的抑制作用  

Inhibitory effect of toosendanin on gastric cancer cells BGC-823 by downregulating circDLST

作　　者：张靖[1] ZHANG Jing(Department of Gastroenterology,Shanghai Sixth People’s Hospital,Shanghai Jiao Tong University,Shanghai 200233,China.)

机构地区：[1]上海交通大学附属第六人民医院消化科,上海200233

出　　处：《上海交通大学学报（医学版）》2020年第9期1202-1206,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金面上项目(81873143);上海市教育委员会高峰高原学科建设计划(20191831)。

摘　　要：目的·探讨川楝素(toosendanin,TSN)通过调节环状RNA circDLST对胃癌细胞BGC-823生长和侵袭的影响。方法·利用不同浓度TSN(0、0.5、1.0和2.0μmol/L)处理胃癌细胞系BGC-823,24 h后定量PCR检测TSN对circDLST表达的影响。将circDLST过表达及其空载对照(CON)慢病毒载体转染胃癌细胞系BGC-823,然后以0.5μmol/L TSN或PBS处理,即将细胞分为circDLST+TSN组、CON+TSN组和CON+PBS组;MTT增殖实验和Transwell侵袭实验观察细胞增殖和侵袭水平的变化。利用circDLST稳转细胞株BGC-823或对照细胞株建立裸鼠皮下移植瘤动物模型,然后每日腹腔注射200μg/kg TSN或等量PBS,即将裸鼠分为circDLST+TSN组、CON+TSN组和CON+PBS组,观察皮下移植瘤的生长情况。结果·与对照组(0μmol/L)比较,3种浓度的TSN均可显著下调circDLST的表达水平(均P<0.01),且呈浓度依赖性。TSN可抑制胃癌细胞增殖和侵袭能力,以及裸鼠皮下的移植瘤生长(均P=0.000),而circDLST过表达可逆转TSN的这些作用(均P<0.01)。结论·TSN可能通过下调circDLST抑制胃癌细胞BGC-823的生长和侵袭。Objective·To investigate the effect of toosendanin(TSN)on the growth and invasion of gastric cancer cells BGC-823 by regulating circDLST expression.Methods·After gastric cancer cells BGC-823 were exposed to different concentrations of TSN(0,0.5,1.0,and 2.0μmol/L)for 24 h,quantitative PCR was used to detect the expression of circDLST.BGC-823 cells were transfected with the circDLST overexpression lentiviral vector or its control vector(CON),and then treated with 0.5μmol/L TSN or PBS.So the cells were divided into circDLST+TSN group,CON+TSN group and CON+PBS group.The viability and invasive potential of BGC-823 cells were observed by MTT proliferation test and Transwell invasion assay.The subcutaneous transplanted tumor models were established by using circDLST-transfected cell line BGC-823 or the control cell line in nude mice,and then 200μg/kg TSN or the same volume of PBS was injected intraperitoneally every day.So the mice were divided into circDLST+TSN group,CON+TSN group and CON+PBS group.Results·Compared with the control group(0μmol/L),all 3 concentrations of TSN decreased the expression levels of circDLST in a concentration dependent manner(P<0.01).TSN could significantly reduce the cell viability,cell invasion and subcutaneous xenograft tumor growth(P=0.000),while circDLST overexpression reversed the inhibitory effect of TSN(P<0.01).Conclusion·TSN may inhibit the growth and invasion of gastric cancer cells BGC-823 by downregulating circDLST expression.

关 键 词：胃癌 川楝素 环状RNA circDLST 肿瘤生长 肿瘤侵袭 

分 类 号：R735.2[医药卫生—肿瘤]