lncRNA BANCR在晶体上皮细胞上皮-间质转化,增殖、凋亡及自噬的作用  

The Effect of lncRNA BANCR on EMT, Proliferation, Apoptosis and Autophagy in Human Lens Epithelial Cells

作　　者：刘含若 白玮玲 夏子尧 董喆 宋旭东 LIU Han-ruo;BAI Wei-ling;XIA Zi-yao;DONG Zhe;SONG Xu-dong(Beiing Tongren Eye Center/Beiing Key Laboratory of Ophthalmology and Visual Sciences/Beijing Institute of Ophthalmology/Beiing Tongren Hospital,Capital Medical University,Beijing,100005,China)

机构地区：[1]首都医科大学附属北京同仁医院北京同仁眼科中心\北京市眼科研究所\眼科学与视觉科学北京市重点实验室,北京100005

出　　处：《现代生物医学进展》2020年第17期3243-3247,3216,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81700813);北京市医院管理局"青苗"计划专项经费(QML20180205);北京市优秀人才培养资助项目;北京市科技新星项目(Z191100001119072);首都医科大学附属北京同仁医院拔尖人才培养计划,医药协同科研创新研究专项(Z181100001918035)。

摘　　要：目的:探索长链非编码RNA BANCR(lncRNA BANCR)在人晶状体上皮细胞FHL24中对上皮-间质转化的作用,并进一步探究了其调控晶体上皮细胞增殖、凋亡及自噬的作用及相关分子机制。方法:运用qReal-time PCR检测TGF-β诱导对FHL24细胞内EMT相关标志物α-SMA,E-cadherin,Coll I,ZO1及BANCR m RNA相对表达量。细胞中转染BANCR。Western印迹检测各组细胞中EMT相关标志蛋白及LC3Ⅱ/Ⅰ的蛋白表达。MTT法检测各组细胞的增殖,凋亡情况。结果:与正常对照组比较,TGF-β诱导组细胞中BANCR,α-SMA, Coll I,ZO1 m RNA的相对表达量明显增加,而E-cadherin m RNA显著下降,差异均有统计学意义(t=-5.031,-7.145,-9.023,-6.012, 5.097均P<0.05),以上因子的蛋白表达趋势相同,差异均有统计学意义(均P<0.05)。si RNA-BANCR TGF-β诱导组细胞中E-cadherin m RNA相对表达量比si RNA TGF-β诱导组显著增加(t=-9.98, P<0.05);α-SMA,Coll I及ZO1 m RNA相对表则显著减少(t=9.003;27.738;19.620, P<0.05)。抑制BANCR后细胞增殖活力48、72 h时细胞活性显著降低(t=5.032, 9.041,均P<0.05),细胞凋亡率显著升(t=16.772,P<0.001)。自噬标志蛋白LC3-II/LC3-I比例增加(P <0.05)。结论:长链非编码BANCR参与了晶体上皮细胞的抑制其上皮-间质转化,抑制BANCR可抑制晶体上皮细胞增殖、增加凋亡及自噬的发生。Objective: To explore the effect of long non-coding RNA BANCR(lncRNA BANCR) on epithelial-mesenchymal transition in human lens epithelial cells FHL24, and to further explore its role in regulating the proliferation, apoptosis and autophagy of lens epithelial cells and related molecular mechanisms. Methods: qReal-time PCR was used to detect the relative expression of EMT-related markers α-SMA, E-cadherin, Coll I, ZO1 and BANCR m RNA induced by TGF-β in FHL24 cells. Transfect the cells with BANCR.Western blotting was used to detect the expression of EMT-related marker proteins and LC3Ⅱ/I in each group of cells. MTT method was used to detect cell proliferation and apoptosis in each group. Results: Compared with the normal control group, the relative expression of BANCR, α-SMA, Coll I, and ZO1 m RNA in the cells of the TGF-β induction group increased significantly, while the E-cadherin m RNA decreased significantly, and the differences were statistically significant(t=-5.031,-7.145,-9.023,-6.012, 5.097 all P<0.05), the protein expression trends of the above factors were the same, and the differences were statistically significant(all P<0.05). The relative expression of E-cadherin m RNA in the cells of the si RNA-BANCR TGF-β induction group was significantly higher than that in the si RNA TGF-β induction group(t=-9.98, P<0.05);the relative expression of α-SMA, Coll I and ZO1 m RNA was significant Decrease(t=9.003;27.738;19.620, P<0.05). After inhibiting BANCR, the cell proliferation activity was significantly reduced at 48 and 72 h(t=5.032, 9.041,all P<0.05), and the apoptosis rate was significantly increased(t=16.772, P<0.001). The ratio of autophagy marker protein LC3-II/LC3-I increased(P<0.05). Conclusions: Long-chain non-coding BANCR is involved in the inhibition of epithelial-mesenchymal transition of lens epithelial cells. Inhibition of BANCR can inhibit the proliferation of lens epithelial cells, increase apoptosis and autophagy.

关 键 词：后发性白内障 长链非编码RNA BANCR 自噬 

分 类 号：R-33[医药卫生] R776.1