重组人癌胚抗原cDNA基因转染小鼠肝癌细胞体外实验模型的建立  

The Establishment of An in Vitro Experimental Model of Murine Hepa Cancer cells Transferred by Recombinant Human CEA Plasmid

作　　者：卢方安[1] 黄冰[2] 杨洁[3] 罗超权[3] 杨英浩[3] 伍新尧[3] 

机构地区：[1]郧阳医学院生化教研室,十堰442000 [2]中山医科大学实验动物中心 [3]中山医科大学生化教研室

出　　处：《郧阳医学院学报》1999年第4期191-193,共3页Journal of Yunyang Medical College

摘　　要：目的：建立表达人癌胚抗原（CEA）的小鼠肝癌细胞模型。方法：用脂质体介导转染法将含人CEA-cDNA的逆转录病毒重组质粒pLXSN-CEA导入小鼠肝癌细胞，利用含G418的选择培养基筛选及放免法测定癌细胞CEA表达量进行鉴定。结果：成功建立表达人CEA的小鼠肝癌细胞模型。结论：利用基因转染技术能将人癌胚抗原cDNA基因导入小鼠肝癌细胞中，并能在选择培养基中生长，且表达人的癌胚抗原。Objective:To establish murine Hepa cancer cell model expressing human carcinoembryonic antigen (CEA).Methods: The recombinant plasmid pLXSN-CEA with human CEAswhd pcoN - CEA with human CEA-cDNA was transferred into the ng lipofecedne. FuIther screening with G4l8 and edng of CEA expressed by the Hepa cancer cells with radio-immunology assay were taken to identify the CEA positive caner cells.Results: The Hepa caner cells model of murine expressing human CEA was obtained successfully. Conclusion:Human CEA - cDNA plasmid could be transferred into murine Hepa cancer cells by DNA transfer techniques.

关 键 词：重组人癌胚抗原 基因转染 肝癌 肝肿瘤 CDNA基因 动物实验 免疫治疗 

分 类 号：R735.7[医药卫生—肿瘤] R－332[医药卫生—临床医学]