Notch1依赖Slug调控乳腺癌的上皮-间质转化过程  

Notch1 mediates breast cancer epithelial-mesenchymal transition through Slug

作　　者：邵珊[1] 安改丽[2] 赵琳[1] 罗敏娜 宁谦[4] 蒙渡[5] 赵新汉[1] 雷建军[6] SHAO Shan;AN Gaili;ZHAO Lin;LUO Minna;NING Qian;MENG Du;ZHAO Xinhan;LEI Jianjun(Department of Oncology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061;Department of Oncology,Shaanxi Provincial People's Hospital,Xi'an 710068;Department of Hematology,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061;Department of Respiratory,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061;Department of Radiotherapy,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061;Department of Hepatobiliary Surgery,The First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]西安交通大学第一附属医院肿瘤内科,陕西西安710061 [2]陕西省人民医院肿瘤内科,陕西西安710068 [3]西安交通大学第一附属医院血液内科,陕西西安710061 [4]西安交通大学第一附属医院呼吸内科,陕西西安710061 [5]西安交通大学第一附属医院肿瘤放疗科,陕西西安710061 [6]西安交通大学第一附属医院肝胆外科,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2020年第6期881-887,共7页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：国家自然科学基金资助项目(No.81502066,No.81702908),陕西省自然科学基础研究计划一般项目(面上)(No.2019JM-115,No.2018JQ8030),西安交通大学基础科研业务费(No.xzy012019090)。

摘　　要：目的探讨Notch1调控乳腺癌上皮-间质转化(EMT)机制。方法通过Jagged1及shNotch1调控Notch信号通路的表达,观察乳腺癌细胞EMT及迁移侵袭情况;通过Slug荧光素酶报告基因,观察Notch1对Slug的报告基因作用。通过调控Slug表达水平,观察Slug在Notch1调控乳腺癌EMT及迁移侵袭中的作用。结果Jagged1活化Notch信号通路后促进乳腺癌的EMT及迁移侵袭过程,运用shRNA敲除Notch1后可逆转Jagged1导致乳腺癌的EMT及迁移侵袭过程,Notch1过表达可激活Slug报告基因,且Slug过表达可逆转shNotch1对EMT的抑制作用。结论Notch1作用于Slug的报告基因,通过Slug调控乳腺癌的EMT及迁移侵袭过程。Objective To investigate the mechanism of Nocth1 in regulating epithelial-mesenchymal transition(EMT)in breast cancer.Methods Jagged1 or shRNA was applied to regulate Notch signaling expression.EMT process and migration and invasion in breast cancer were determined.Moreover,Slug luciferase reporter assay was used to investigate the effect of Notch1 on Slug expression.And Slug expression was regulated by shRNA.The effects of Slug on Notch1 mediated breast cancer EMT process and migration and invasion were determined.Results Jagged1 activated Notch signaling and promoted EMT process and migration and invasion in breast cancer.Overexpression of Notch1 could induce Slug promoter activation,and Slug overexpression could reverse the inhibition of Notch1 shRNA on breast cancer s EMT process and migration and invasion.Conclusion Notch1 regulates EMT process,migration and invasion in breast cancer through targeting the Slug promoter.

关 键 词：乳腺癌 Notch1信号通路 SLUG 上皮-间质转化(EMT) 侵袭 

分 类 号：R737.9[医药卫生—肿瘤]