重金属铜胁迫下小珊瑚藻差异蛋白的双向电泳分析  

Analysis of Soluble Proteins in Corallina pilulifera under Heavy Metal Cu^2+ Stress Using Two-dimensional Electrophoresis

作　　者：王小蓓 刘庆 王秀静[1] 李丽霞[1] WANG Xiao-bei;LIU Qing;WANG Xiu-jing;LI Li-xia(School of Life Sciences,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2020年第4期495-500,共6页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家自然科学基金资助项目(31300326).

摘　　要：本研究以小珊瑚藻为试材,设置不同浓度梯度的Cu^2+(0.25 mg/L、0.5 mg/L、1.0 mg/L),提取藻体蛋白进行双向电泳,从蛋白质水平研究Cu^2+离子胁迫对小珊瑚藻的影响,为小珊瑚藻抗铜机制的探究及研发海洋环境的监测途径提供一定的理论依据.结果显示:在Cu^2+胁迫下,小珊瑚藻藻体蛋白表达发生变化,体现出明显的“低促高抑”效应,其中0.25 mg/L Cu^2+处理组变化尤为显著,差异大于2.5倍的显著差异蛋白点有49个;进一步选取7个差异蛋白点进行质谱分析,成功鉴定出4个阳性结果,分别为ABC转运蛋白、超氧化物歧化酶、叶绿素外在蛋白及保守假定蛋白.Three concentrations of Cu^2+(0.25 mg/L,0.5 mg/L and 1.0 mg/L)are used to test the effects of Cu^2+on Corallina pilulifera at protein level using two-dimensional electrophoresis.It provides a theoretical basis for the exploration of copper resistance mechanism of C.pilulifera and the research and development of monitoring methods of marine environment.The results show that under Cu^2+stress,protein expression changes obviously,reflecting the“low dose promoting and high dose inhibiting”effect.Significant changes in protein expression are observed in the treatment of 0.25 mg/L Cu^2+,with 49 spots show more than 2.5-fold changes in abundance.Seven of the 49 spots are selected for further study using mass spectrometry analysis.Four proteins are identified and confirmed to be ABC transporters,superoxide dismutase(SOD),chlorophyll external protein and hypothetical protein,respectively.

关 键 词：重金属 铜胁迫 小珊瑚藻 双向电泳 差异蛋白 

分 类 号：X145[环境科学与工程—环境科学]