LncRNA LINC01138靶向miR-193a-3p对卵巢癌A2780细胞增殖、迁移、侵袭和凋亡的影响  

Effects of LncRNA LINC01138 targeted miR-193a-3p on proliferation,migration,invasion and apoptosis of ovarian cancer A2780 cells

作　　者：晁宏图 张孟丽 CHAO Hongtu;ZHANG Mengli(Department of Gynecologic Oncology,Henan Tumor Hospital(the Affiliated Cancer Hospital,Zhengzhou University),Zhengzhou 450003)

机构地区：[1]河南省肿瘤医院(郑州大学附属肿瘤医院)妇瘤科,郑州450003

出　　处：《郑州大学学报（医学版）》2020年第5期668-672,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省科技攻关项目(172102310301)。

摘　　要：目的:探讨长链非编码RNA LINC01138(LncRNA LINC01138)通过吸附微小RNA-193a-3p(miR-193a-3p)调控其表达,对卵巢癌细胞增殖、迁移、侵袭与凋亡的影响。方法:采用qRT-PCR法检测40例卵巢癌及相应癌旁正常卵巢组织、正常卵巢上皮细胞(HOSE)及3种卵巢癌细胞(A2780、SKOV3、OVCR3)中LINC01138与miR-193a-3p的表达水平。分别将si-con、si-LINC01138、si-LINC01138与anti-miR-con、si-LINC01138与anti-miR-193a-3p转染至A2780细胞,转染后48 h采用MTT法、Transwell小室实验分别检测细胞增殖、迁移及侵袭能力,Annexin V-FITC/PI染色后采用流式细胞术检测细胞凋亡率。结果:与癌旁正常卵巢组织相比,卵巢癌组织中LINC01138的表达升高,miR-193a-3p的表达降低(P<0.001);与正常卵巢上皮细胞HOSE比较,卵巢癌细胞A2780、SKOV3、OVCR3中LINC01138的表达升高,miR-193a-3p的表达降低(P<0.05),其中LINC01138在卵巢癌A2780细胞中的表达相对较高,选用卵巢癌A2780细胞进行后续实验。与si-con组比较,si-LINC01138组细胞增殖、迁移及侵袭能力降低,细胞凋亡率升高(P<0.05);与si-LINC01138+anti-miR-con组比较,si-LINC01138+anti-miR-193a-3p组细胞活力增强,迁移及侵袭细胞数增加,细胞凋亡率降低(P<0.05)。结论:抑制LINC01138可通过上调miR-193a-3p的表达进而减弱卵巢癌A2780细胞增殖、迁移、侵袭能力,诱导细胞凋亡。Aim:To investigate the effects of long-chain non-coding RNA LINC01138(LncRNA LINC01138)on the proliferation,migration,invasion and apoptosis of ovarian cancer cells by absorbing microRNA-193a-3p(miR-193a-3p).Methods:The expression levels of LINC01138 and miR-193a-3p in 40 cases of ovarian cancer and corresponding normal ovarian tissue,normal ovarian epithelial cells(HOSE)and 3 kinds of ovarian cancer cells(A2780,SKOV3,OVCR3)were detected by qRT-PCR.si-con,si-LINC01138,si-LINC01138 and anti-miR-con,si-LINC01138 and anti-miR-193a-3p were transfected into A2780 cells,respectively.After 48 hours,MTT method and Transwell cell experiment were used to detect cell proliferation,migration and invasion ability.Flow cytometry was used to detect the apoptosis rate after Annexin V-FITC/PI staining.Results:Compared with normal ovarian tissue adjacent to the cancer,the expression of LINC01138 increased in ovarian cancer tissue,and the expression of miR-193a-3p decreased(P<0.001).Compared with normal ovarian epithelial cells HOSE,the expression of LINC01138 in ovarian cancer cells A2780,SKOV3 and OVCR3 increased,and the expression of miR-193a-3p decreased(P<0.05).The expression level of LINC01138 in A2780 cells was relatively high,and A2780 cells were used for subsequent experiments.Compared with the si-con group,the si-LINC01138 group′s cell proliferation,migration,and invasion ability reduced,and the apoptosis rate increased(P<0.05).Compared with the si-LINC01138+anti-miR-con group,the cell viability increased in the si-LINC01138+anti-miR-193a-3p group,the number of migrating and invasive cells increased,and the apoptosis rate decreased(P<0.05).Conclusion:Inhibition of LINC01138 could attenuate the proliferation,migration and invasion of ovarian cancer cells A2780 and induce apoptosis by up-regulating the expression of miR-193a-3p.

关 键 词：LncRNA LINC01138 miR-193a-3p 卵巢癌 A2780细胞 增殖 侵袭 凋亡 

分 类 号：R737.31[医药卫生—肿瘤]