基于微流控技术的抗生素敏感性分析研究进展  

Advances in antibiotic sensitivity analysis based on microfluidics

作　　者：阿里亚·阿不力米提 孙凯 伊佐斯莫娃·奥克萨 李哲煜 ALIYA Abulimiti;SUN Kai;IZOSIMOVA SOksana;LI Zheyu(State Key Laboratory of Urban Water Resource and Environment,School of Environment,Harbin Institute of Technology,Harbin 150090;Institute of Earth Sciences,Saint-Petersburg State University,Saint-Petersburg 199034)

机构地区：[1]哈尔滨工业大学环境学院,城市水资源与水环境国家重点实验室,哈尔滨150090 [2]圣彼得堡国立大学地球科学研究所,圣彼得堡,俄罗斯联邦199034

出　　处：《环境科学学报》2020年第10期3565-3573,共9页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.61674046);城市水资源与水环境国家重点实验室项目(No.2017TS04)。

摘　　要：微流控技术是一种基于对微纳流体的操控从而实现特定功能的分析技术,具有微型化、集成化等特点,在生物、化学、医学等领域表现出了很好的发展潜力和应用前景.利用微流控芯片开发细菌培养和抗生素敏感性分析技术,既能对细菌的培养条件实现精确调控,对细菌生长状态进行实时观察,还能显著节省培养时间和使用成本,并缩短抗生素最小抑制浓度的检测周期.有鉴于此,本文针对抗生素敏感分析中微流控技术的研究进展进行评述,以期为相关研究提供一些思路.Microfluidic technology is a technique that uses microchannels to process micro⁃fluidics.Microfluidic technology has the characteristic of miniaturization and integration and has great development potential and application prospects in the fields of biology,chemistry and medicine.In terms of bacterial culture and antibiotic sensitivity analysis,microfluidic technology can not only accurately control the culture conditions and real⁃time observe the growth of bacteria,but also greatly reduce the cost and time of culture and shorten the detection cycle of the minimum inhibitory concentration of antibiotics.This paper introduces several microfluidic chips for bacterial culture,and summarizes the application progress of microfluidic technology in antibiotic sensitivity analysis,hoping to provide some ideas for the design and application of new microfluidic platform.

关 键 词：微流控芯片 细菌培养 抗生素 最小抑制浓度(MIC) 抗生素敏感性测试(AST) 

分 类 号：X172[环境科学与工程—环境科学]