A novel CCCH-type zinc finger protein SAW1 activates OsGA20ox3 to regulate gibberellin homeostasis and anther development in rice  

作　　者：Bin Wang Ruiqiu Fang Faming Chen Jingluan Han Yao-Guang Liu Letian Chen Qinlong Zhu 

机构地区：[1]State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,South China Agricultural University,Guangzhou 510642,China [2]Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China [3]Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms,South China Agricultural University,Guangzhou 510642,China [4]College of Life Sciences,South China Agricultural University,Guangzhou 510642,China [5]Dongyang Institute of Maize Research,Zhejiang Academy of Agricultural Sciences,Dongyang 322100,China

出　　处：《Journal of Integrative Plant Biology》2020年第10期1594-1606,共13页植物学报（英文版）

基　　金：We thank the lnstrumental Analysis&Research Center of South China Agricultural University for SEM and TEM analyses.This research was supported bygrants from the National Natural Science Foundationof China(31600242);the Natural Science Foundationof Guangdong(2017A030313184,2016A030310443);the China Postdoctoral ScienceFoundation(2016M592501,2016M592500);the Major Pro-gram of Guangdong Basic and Applied Research(2019B030302006);The authors declare no competing financial interests.

摘　　要：Male sterility is a prerequisite for hybrid seed production.The phytohormone gibberellin(GA)is in-volved in regulating male reproductive development,but the mechanism underlying GA homeostasis in anther development remains less understood.Here,we report the isolation and characterization of a new positive regulator of GA homeostasis,swollen anther wall 1(SAW1),for anther development in rice(Oryza sativa L.).Rice plants carrying the recessive mutant allele saw1 produces abnormal anthers with swollen anther wall and aborted pollen.Clustered regularly interspaced short palindromic repeats(CRISPR)/CRIPSR-associated protein 9-mediated knockout of SAW1 in rice generated similar male sterile plants.SAW1 encodes a novel nucleus-localizing CCCH-tandem zinc finger protein,and this protein could directly bind to the promoter region of the GA synthesis gene OsGA20ox3 to induce its anther-specific expression.In the saw1 anther,the significantly decreased OsGA20ox3 expression resulted in lower bio-active GA content,which in turn caused the lower expression of the GA-inducible anther-regulator gene OsGAMYB.Thus,our results disclose the mechanism of the SAW1-GA20ox3-GAMYB pathway in controlling rice anther development,and provide a new target gene for the rapid generation of male sterile lines by genome editing for hybrid breeding.

关 键 词：STERILE HOMEOSTASIS ANTHER 

分 类 号：S511[农业科学—作物学]