盐酸羟考酮负调控LncRNA LINC01857抑制宫颈癌细胞Siha增殖、迁移及侵袭  

Oxycodone hydrochloride negatively regulated LncRNA LINC01857 to inhibit the proliferation,migration and invasion of cervical cancer cell Siha

作　　者：张秀双[1] 徐铭军[1] 车向明[1] 曹秀玲[1] 李晓光[1] ZHANG Xiushuang;XU Mingjun;CHE Xiangming;CAO Xiuling;LI Xiaoguang(Anesthesiology Dept,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing,China,100006)

机构地区：[1]首都医科大学附属北京妇产医院麻醉科,北京100006

出　　处：《分子诊断与治疗杂志》2020年第10期1392-1396,共5页Journal of Molecular Diagnostics and Therapy

基　　金：北京市卫生和计划生育委员会科技成果和适宜技术推广项目(2018-TG-21)。

摘　　要：目的探讨盐酸羟考酮是否通过负向调控LncRNA LINC01857而抑制宫颈癌细胞Siha增殖、迁移及侵袭。方法采用不同浓度的盐酸羟考酮处理人宫颈癌细胞Siha,分别将si-NC、si-LINC01857转染至Siha细胞,分别将pcDNA、pcDNA-LINC01857转染至Siha细胞后使用盐酸羟考酮处理;采用qRT-PCR法检测LINC01857的表达量;采用MTT实验检测细胞增殖;采用平板克隆形成实验检测克隆形成数;Transwell小室实验检测细胞迁移及侵袭;Western blot法检测E-cadherin、N-cadherin蛋白表达量。结果与对照组比较,盐酸羟考酮处理后可明显降低LINC01857的表达水平、OD值及N-cadherin蛋白水平,提高E-cadherin蛋白水平,差异有统计学意义(P<0.05);与转染si-NC比较,转染si-LINC01857后OD值及N-cadherin蛋白水平降低,克隆形成数、迁移及侵袭细胞数减少,E-cadherin蛋白水平升高,差异有统计学意义(P<0.05)。结论盐酸羟考酮通过下调LINC01857的表达从而减弱宫颈癌细胞增殖、迁移及侵袭能力。Objective To explore whether oxycodone hydrochloride could inhibit the proliferation,migration and invasion of cervical cancer cell Siha through negative regulation of LncRNA LINC01857.Methods Different concentrations of oxycodone hydrochloride were used to treat human cervical cancer cell Siha,and si-NC,si-LINC01857 were respectively transfected into Siha cells.After transfecting pcDNA and pcDNA-LINC01857 into Siha cells,they were treated with oxycodone hydrochloride.The qRT-PCR method was used to detect the expression of LINC01857.MTT assay was used to detect cell proliferation.The plate colony formation experiment was used to detect the number of colonies formed.The Transwell chamber test was used to detect cell migration and invasion.Western blot method was used to detect the expression of E-cadherin and N-cadherin protein.Results Compared with the control group,oxycodone hydrochloride treatment could significantly reduce the expression level of LINC01857,OD value and the protein level of N-cadherin,and increase the protein level of E-cadherin,the difference was statistically significant(P<0.05).Compared with the transfection of si-NC,the OD value and the protein level of N-cadherin were decreased after si-LINC01857 transfection,the number of clone formation,migration and invasion cells were decreased,and the protein level of E-cadherin was increased,the difference was statistically significant(P<0.05).Conclusion Oxycodone hydrochloride reduced the proliferation,migration and invasion of cervical cancer cells by down-regulating the expression of LINC01857.

关 键 词：盐酸羟考酮 LncRNA LINC01857 宫颈癌 增殖 迁移 侵袭 

分 类 号：R737.33[医药卫生—肿瘤]