MDR1基因外显子26多态性与儿童急性淋巴细胞白血病易感性的关系  

Relationship between MDR1 gene exon 26 polymorphism and susceptibility to childhood acute lymphoblastic leukemia

作　　者：谢昕 史利欢 陈静[1] 刘炜[1] XIE Xin;SHI Lihuan;CHEN Jing;LIU Wei(Henan Key Laboratory of Pediatric Hematology,Children's Hospital Affiliated of Zhengzhou University/Henan Children's Hospital/Zhengzhou Children's Hospital,Zhengzhou 450000,China)

机构地区：[1]郑州大学附属儿童医院/河南省儿童医院/郑州儿童医院河南省小儿血液医学重点实验室,河南郑州450000

出　　处：《临床医学研究与实践》2020年第31期104-105,108,共3页Clinical Research and Practice

摘　　要：目的探讨MDR1基因外显子26多态性与儿童急性淋巴细胞白血病易感性的关系。方法采用整群随机抽样法选取我院2017年2月至2019年6月收治的90例急性淋巴细胞白血病患儿,设为研究组。同期以年龄、性别等为配比标准,根据1∶1比例进行配对,选取90例健康体检儿童作为对照组。采取多重聚合酶链-限制性片段长度多态性(PCR-RFLP)技术检测两组研究对象的MDR1第26位点基因型,分析MDR1基因外显子26多态性与儿童急性淋巴细胞白血病易感性之间的关系。结果研究组的MDR1基因缺失型占比高于对照组,非缺失型占比低于对照组,差异具有统计学意义(P<0.05)。研究组患儿的等位基因发生频率:MDR1基因型中C3435T位点C与T发生频率分别为37.10%和62.90%,其中CC基因型发生频率为29.03%,TT基因型发生频率为54.84%,CT基因型发生频率为16.13%。结论MDR1基因缺失的儿童急性淋巴细胞白血病易感性更高,基因型TT与等位基因T可能是儿童急性淋巴细胞白血病发生的易感因素。Objective To investigate the relationship between MDR1 gene exon 26 polymorphism and susceptibility to childhood acute lymphoblastic leukemia.Methods A total of 90 children with acute lymphoblastic leukemia admitted in our hospital from February 2017 to June 2019 were selected by cluster random sampling method and set as study group.At the same time,90 healthy children were selected as control group according to the age,gender and the ratio of 1∶1.Multiple polymerase chain restriction fragment length polymorphism(PCR-RFLP)was used to detect the MDR126th locus genotype of the two groups of study objects.The relationship between MDR1 gene exon 26 polymorphism and susceptibility to childhood acute lymphoblastic leukemia were analyzed.Results The proportion of children with MDR1 gene deletion in the study group was higher than that in the control group,and the proportion of non-deletion type was lower than that in the control group,and the differences were statistically significant(P<0.05).The frequency of allele in the study group:in MDR1 genotype,the frequency of C3435T locus C and T was 37.10%and 62.90%,respectively;the frequency of CC genotype,TT genotype and CT genotype were 29.03%,54.84%and 16.13%,respectively.Conclusion Children with MDR1 gene deletion are more susceptible to acute lymphoblastic leukemia.Genotype TT and allele T may be the susceptible factors of childhood acute lymphoblastic leukemia.

关 键 词：儿童急性淋巴细胞白血病 MDR1基因 外显子26多态性 易感性 

分 类 号：R733.7[医药卫生—肿瘤]