Lin28a对大鼠移植静脉内膜增生影响的实验研究  

Effect of Lin28a on intimal hyperplasia of transplanted vein in rats

作　　者：万树成 曹辉[1] 李楠[1] 曹灿 李震[1] Wan Shuwei;Cao Hui;Li Nan;Cao Can;Li Zhen(Department of Endovascular surgery,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院腔内血管外科,郑州450052

出　　处：《中华血管外科杂志》2020年第3期202-205,共4页Chinese Journal of Vascular Surgery

基　　金：国家自然科学基金(81600377)。

摘　　要：目的探讨Lin28a对大鼠移植静脉内膜增生的影响。方法48只雄性Sprague-Dawley大鼠均构建静脉移植模型。将大鼠随机分为移植未处理组(移植后不做处理)、凝胶组(移植后在静脉外膜涂抹凝胶)、阴性对照组(移植后在静脉外膜涂抹阴性对照病毒和凝胶的混悬液)、Lin28a-shRNA组(移植后在静脉外膜涂抹Lin28a-shRNA慢病毒和凝胶的混悬液),每组12只。术后14 d和28 d取移植静脉,行HE染色,计算内膜厚度(I)和中膜厚度(M),应用免疫组织化学染色方法检测α-平滑肌肌动蛋白(α-SMA)和增殖细胞核抗原(PCNA)的表达水平。结果(1)内膜厚度:术后14 d及28 d,Lin28a-shRNA组内膜厚度分别为(19.82±2.10)μm及(56.61±3.17)μm,I/M值分别为0.15±0.01及0.68±0.03;Lin28a-shRNA组的内膜厚度及I/M值与移植未处理组、凝胶组和阴性对照组比较均降低,差异均有统计学意义(P均<0.05)。(2)α-SMA检测结果:术后28 d,Lin28a-shRNA组移植静脉α-SMA光密度值为(25.66±1.36)×10-3,与移植未处理组、凝胶组和阴性对照组比较,差异有统计学意义(P均<0.05)。(3)PCNA阳性表达水平:术后14 d及28 d,Lin28a-shRNA组PCNA阳性表达率分别降低(24.82±4.74)%及(11.67±3.02)%,与移植未处理组、凝胶组和阴性对照组比较,差异有统计学意义(P均<0.05)。结论降低Lin28a的表达能够抑制大鼠移植静脉内膜增生。Objective To investigate the effect of lin28a on intimal hyperplasia of vein grafts in rats.Methods A total of 48 male Sprague Dawley rats were used to construct vein transplantation model.The rats were randomly divided into 4 groups:untreated group(no treatment after transplantation),gel group(gel was applied to the vein adventitia after transplantation),negative control group(suspension of negative control virus and gel was applied to the vein adventitia after transplantation)and lin28a-shRNA group(suspension of lin28a shRNA lentivirus and gel was applied to the vein adventitia after transplantation),12 rats in each group.On the 14th and 28th day after operation,the vein grafts were taken and stained with hematoxylin eosin(HE).The intimal thickness(I)and middle membrane thickness(M)were calculated.The expression levels ofα-smooth muscle actin(α-SMA)and proliferating cell nuclear antigen(PCNA)were detected by immunohistochemistry.Results(1)On the 14th day after operation,the intimal thickness of lin28a shRNA group was(19.82±2.10)μm and the I/M was 0.15±0.01;the intimal thickness and the I/M of lin28a shRNA group were both significantly lower than those of the untreated group,gel group and negative control group(all P<0.05).On the 28th day after operation,the intimal thickness of lin28a shRNA group was(56.61±3.17)μm and the I/M was 0.68±0.03;the intimal thickness and the I/M of lin28a shRNA group were both significantly lower than those of the other three groups(all P<0.05).(2)On the 28th day after operation,the optical density ofα-SMA in lin28a shRNA group was(25.66±1.36)×10-3,which was significantly lower than that of the other three groups(all P<0.05).(3)On the 14th day after operation,the positive expression rate of PCNA in lin28a shRNA group was(24.82±4.74)%,which was significantly lower than that of the other three groups(all P<0.05).On the 28th day after operation,the positive expression rate of PCNA in lin28a shRNA group was(11.67±3.02)%,which was significantly lower than that of the other th

关 键 词：Lin28a 静脉移植 内膜增生 

分 类 号：R73[医药卫生—肿瘤]