淫羊藿苷与补肾固齿丸对大鼠慢性牙周炎牙槽骨重建的比较研究  

Comparative study on icariin and Bu-Shen-Gu-Chi-Wan for alveolar reconstruction in chronic periodontitis in rats

作　　者：廖丹 葛颂 LIAO Dan;GE Song(Affiliated Stomatological Hospital of Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学附属口腔医院,贵州遵义563000

出　　处：《口腔疾病防治》2020年第12期759-767,共9页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金项目(81860197)。

摘　　要：目的观察淫羊藿苷(icariin,ICA)与补肾固齿丸改善大鼠慢性牙周炎牙槽骨吸收情况,探讨ICA用于治疗慢性牙周炎的效果及可能机制。方法建立大鼠牙周炎模型后,随机分为慢性牙周炎组(P组)、ICA低剂量组(L组)、ICA高剂量组(H组)以及补肾固齿丸组(B组)。各组分别给药1个月、2个月,通过检测血清骨转换标志物血清骨钙素(osteocalcin,OCN),同时采用显微CT(micro computed tomography,Micro-CT)扫描后三维重建,检测特定位点骨参数及记录釉-牙骨质界-牙槽嵴顶(cement-to-enamel junction-alveolar bone crest,CEJ-ABC)的距离为牙槽骨吸收值,组织学切片评价ICA对大鼠实验性牙周炎牙槽骨修复重建的影响。结果H组、L组及B组与P组相比,血清OCN均有所降低(P<0.05);H组、L组及B组的骨体积分数、骨小梁数目较P组明显增大(P<0.05)。H组、B组与P组相比,骨小梁厚度明显增大(P<0.05),骨小梁分离度明显减小(P<0.05);L组相应骨参数变化趋势与H组一致,但仅在给药2个月时骨小梁分离度与P组差异有统计学意义(P<0.05)。对牙槽骨吸收值测量分析,相较于P组,L组、H组、2个月时的B组CEJ-ABC均明显降低,差异有统计学意义(P<0.05)。结论ICA与补肾固齿丸能改善实验性大鼠慢性牙周炎的牙槽骨吸收情况,其机制可能与调节血清骨钙素水平有关。Objective To observe the effects of icariin(ICA)and Bu-Shen-Gu-Chi-Wan on the alveolar bone ab-sorption of chronic periodontitis in rats,and to explore the effect and possible mechanism of ICA in the treatment of chronic periodontitis.Methods After the establishment of the periodontitis model,the rats were divided into the peri-odontitis group(group P),ICA high dose group(group H),ICA low dose group(group L)and Bu-Shen-Gu-Chi-Wan treat-ment group(group B).Each group received treatment for one month and two months,separately,and the serum osteocal-cin(OCN)level was measured.Three-dimensional reconstruction was performed after micro computed tomography(mi-cro CT)scanning to measure the bone parameters of specific points,and the distance between the enamel cementum boundary and alveolar crest(CEJ-ABC)was recorded as alveolar bone resorption value.Tissue sections were generated to evaluate the effect of ICA on alveolar bone repair and reconstruction in rats with experimental periodontitis.Re-sults Compared with the periodontitis group(group P),OCN levels in the serum in treatment groups(groups H,L and B)were decreased significantly(P<0.05);the values of bone volume/tissue volume(BV/TV)and trabecular number(Tb.N)in treatment groups(groups H,L and B)were significantly higher than that in group P(P<0.05).Compared with group P,trabecular thickness(Tb.Th)values of groups H and B significantly increased,and trabecular separation(Tb.Sp)of groups H and B significantly decreased(P<0.05).The changing trend of parameters in group L was the same as that in group H but only after two months of administration.The difference between Tb.Sp values in groups L and P was statistically significant(P<0.05).Compared with group P,the CEJ-ABC distance significantly reduced in group L(1 month and 2 months after administration),group H(1 month and 2 months after administration),and group B(only 2 months after administration)(P<0.05).Conclusion ICA and Bu-Shen-Gu-Chi-Wan improve the alveolar bone resorp-tion in an experimental model of chronic

关 键 词：淫羊藿苷 补肾固齿丸 实验性牙周炎 牙槽骨修复 骨钙素 显微CT 骨体积分数 骨小梁数 骨小梁分离度 牙槽骨吸收 

分 类 号：R781.4[医药卫生—口腔医学]