泛素化修饰及其在植物蛋白质组学中的研究进展  

Ubiquitin Modification and Its Research Progress in Plant Proteomics

作　　者：张嘉麟 李海英 Zhang Jialin;Li Haiying(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang University,Harbin 150500;Key Laboratory of Molecular Biology,College of Heilongjiang Province,School of Life Sciences,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500 [2]黑龙江大学生命科学学院,黑龙江省普通高校分子生物学重点实验室,哈尔滨150080

出　　处：《中国农学通报》2020年第30期75-81,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金“甜菜M14品系抗氧化酶系统响应盐胁迫应答过程的研究”(31471552);黑龙江省高校创新团队建设计划项目“寒区植物重要基因资源的挖掘与种质创新”(2014TD004)。

摘　　要：泛素化修饰是真核生物中一种重要的蛋白质翻译后修饰,几乎参与调控细胞内所有重要的生命活动过程。近年来,随着泛素化蛋白质富集技术和质谱技术的不断发展,泛素化蛋白质组学研究取得了重大进展。目前泛素化蛋白质组学研究多集中在动物和酵母中,而在植物中开展的相对较少。为了总结植物中泛素化蛋白质组学的最新研究成果,本文对泛素化修饰、质谱法鉴定泛素化位点、泛素化蛋白的富集方法及泛素化蛋白质组学在植物光调节、激素处理和逆境胁迫条件下的应用研究进行综述,为泛素化修饰在植物领域的最新研究提供理论基础。Ubiquitination modification is an important post-translational modification of proteins, which participates in the regulation of many important life activities in cells. In recent years, with the development of ubiquitinated proteins enrichment technology and mass spectrometry technology, great progress has been made in ubiquitination proteomics. At present, there are many ubiquitination proteomics studies in animals and yeasts, but few in plants. In order to summarize the latest research results of ubiquitination proteomics in plants, the ubiquitination modifications, the identification of ubiquitination sites by mass spectrometry, the enrichment methods of ubiquitination proteins and the application of ubiquitination proteomics in plant light regulation, hormone treatment and stress conditions were reviewed in this paper, which could provide the rationale for the latest research of ubiquitination modifications in plants.

关 键 词：泛素化修饰 泛素化蛋白的富集 质谱 植物蛋白质组学 

分 类 号：S184[农业科学—农业基础科学]