飞燕草素抑制大鼠乳腺癌发生的机制探索  

Mechanism of Delphinidin Suppressing Breast Carcinogenesis in Rats

作　　者：白金玉 宋慧敏 徐苗 徐嘉琪 韩彬 彭晓莉 Bai Jinyu;Song Huimin;Xu Miao;Xu Jiaqi;Han Bin;Peng Xiaoli(School of Public Health,Chengdu Medical College,Chengdu 610500,Sichuan,China)

机构地区：[1]成都医学院公共卫生学院,成都610500

出　　处：《肿瘤预防与治疗》2020年第10期830-835,共6页Journal of Cancer Control And Treatment

基　　金：国家自然科学基金(编号:81402675);四川省教育厅课题(编号:17ZA0114);成都医学院大学生创新项目(编号:201713705074);成都医学院校基金团队项目(编号:CYTD17-04)。

摘　　要：目的:本课题组前期研究发现飞燕草素能显著抑制甲基亚硝基脲(1-methyl-1-nitrosourea,MNU)诱导的大鼠乳腺癌发生,本研究初步探讨飞燕草素抑制乳腺癌发生的机制。方法:免疫组化染色检测致癌物诱导大鼠乳腺组织的雌激素受体-α(estrogen receptor-α,ER-α)水平;Western blot检测飞燕草素对乳腺癌MCF-7细胞的ER-α、G蛋白偶联雌激素受体(G protein coupled estrogen receptor,GPER)、Notch-1和10号染色体缺失的磷酸酶(phosphataseand tensin homologue deleted on chromosome,PTEN)表达情况的影响。结果:飞燕草素干预能明显降低MNU诱导大鼠乳腺组织ER-α的表达,体外实验发现飞燕草素能够抑制MCF-7细胞ER-α、GPER蛋白及Notch-1蛋白表达水平,同时还能够提高PTEN蛋白表达水平。结论:飞燕草素能通过抑制ER-α、GPER蛋白表达及调节Notch-1/PTEN信号通路发挥抑制乳腺癌的作用。Objective:Our previous study observed that delphinidin can effectively suppress 1-methyl-1-nitrosourea(MNU)-induced breast carcinogenesis.The present study aims to elucidate the antineoplastic effect of delphinidin on breast cancer and its underlying mechanisms.Methods:Immunohistochemistry was performed to detect estrogen receptor-α(ER-α)expression in breast tissues of rats at the end of the experiments.Western blot was conducted to analyze the effect of delphinidin on the expression of ER-α,G protein-coupled estrogen receptor(GPER),Notch-1 and phosphatase and tensin homologue deleted on chromosome(PTEN)in breast cancer MCF-7 cells.Results:Delphinidin decreased the expression of ER-αin breast tissues of MNU-treated rats,also decreased the expression of ER-α,GPER and Notch-1 in MCF-7 cells,and increased the expression of PTEN in MCF-7 cells.Conclusion:Delphinidin can suppress breast carcinogenesis through inhibiting ER-αand GPER expression and Notch-1/PTEN signalling pathway.

关 键 词：飞燕草素 乳腺癌 雌激素受体-Α G蛋白偶联雌激素受体 Notch-1/PTEN信号通路 

分 类 号：R737.9[医药卫生—肿瘤] R966[医药卫生—临床医学]