CUEDC1在急性髓系白血病(非M3型)患者中的预后意义  

Prognostic Significance of CUEDC1 in Patients with Acute Myeloid Leukemia(non-M3)

作　　者：庄万传[1] 吴庆运 孟凡静 徐开林 ZHUANG Wan-Chuan;WU Qing-Yun;MENG Fan-Jing;XU Kai-Lin(Department of Hematology,The Second People’s Hospital of Lianyungang,Lianyungang 222023,Jiangsu Province,China;Blood Diseases Institute,The Affiliated Hospital of Xuzhou Medical University,Xuzhou 221002,Jiangsu Province,China)

机构地区：[1]连云港市第二人民医院血液科,江苏连云港222023 [2]徐州医科大学血液病研究所,江苏徐州221002

出　　处：《中国实验血液学杂志》2020年第5期1464-1467,共4页Journal of Experimental Hematology

基　　金：江苏省卫生计生委资助项目(QNRC2016495);连云港市第五期"521高层次人才培养工程"科研资助项目。

摘　　要：目的:探讨CUEDC1在非M3型急性髓系白血病(acute myeloid leukemia,AML)患者中的预后意义。方法:选取52例初诊AML(非M3型)患者纳入AML(非M3)组,选取同期10例缺铁性贫血患者纳入对照组,从患者骨髓中分离骨髓单个核细胞(bone marrow mononuclear cells,BMMC),采用实时荧光定量PCR(RT-PCR)检测CUEDC1的表达水平。比较各亚型AML患者BMMC中CUEDC1 mRNA的表达水平;根据CUEDC1 mRNA表达水平将初诊AML患者分为低表达组及高表达组,第一疗程化疗结束后比较初次诱导治疗完全缓解率,并采用回顾性分析比较缓解组和未缓解组CUEDC1 mRNA相对表达水平。结果:CUEDC1在初诊的52例AML(非M3型)各亚型及10例缺铁性贫血患者BMMC中均表达,但在AML患者组的表达水平高于对照组(P<0.05),且在急性单核细胞白血病(M5)患者中的表达水平最高(P<0.05);CUEDC1低表达的AML患者第一疗程诱导完全缓解率(76.2%,16/21)高于高表达的AML患者缓解率(67.7%,21/31),但差异无统计学意义(P>0.05);初诊AML(非M3型)缓解组CUEDC1 mRNA表达水平低于未缓解组(P<0.05)。结论:CUEDC1在AML初诊患者BMMC中高表达,且在急性单核细胞白血病(M5)患者BMMC中的表达最高,初诊AML患者CUEDC1的表达水平可能与其预后相关。Objective:To investigate the prognostic significance of CUEDC1 in patients with acute myeloid leukemia(non-M3).Methods:52 cases newly diagnosed AML(non-M3)were selected and enrolled in AML non-M3 group,at the same time,10 cases of iron doficiency anemia were selected and enrolled in control group.The bone marrow mononuclear cells(BMMC)were isolated from bone marrow of patients,the expression level of CUEDC1 in BMMC was detected by RT-PCR,the expression level of CUEDC1 mRNA in BMMC of AML-subtype patients was compared.The AML patients were divided into low and high expression groups according to the expression level of CUEDC1 mRNA,and the complete remission rate after the first chemothrapy course was compared,and the relative expression level of CUEDC1 mRNA between the remission and the non-remission group were compared.Results:CUEDC1 was expressed in BMMC of 52 newly diagnosed patients with AML(non-M3)of all subtypes,which was higher than that in control group(P<0.05),and the expression level of CUEDC1 mRNA in M5 patients was the highest(P<0.05).In CUEDC1 low expression group,induced complete remisson rate(76.2%,16/21)after the first course of treatment seemed higher than that of the high expression group(67.7%,21/31),but the difference was not statistically significant;the expression level of CUEDC1 mRNA in the remission group of patients with newly diagnosed AML(non-M3)was lower than that in the non-remission group(P<0.05).Conclusion:CUEDC1 is highly expressed in newly diagnosed patients with AML,among which the CUEDC1 mRNA expression level in M5 patients is the highest,the expression of CUEDC1 mRNA possibly relates to the prognosis of patients with AML.

关 键 词：CUEDC1 急性髓系白血病 实时荧光定量PCR 预后 

分 类 号：R733.71[医药卫生—肿瘤]