美托洛尔对大鼠腹腔暴露后肠黏膜屏障的影响  

Effect of metoprolol on intestinal mucosal barrier in rats after peritoneal air exposure

作　　者：谈善军[1] 孟庆洋[1] 庄秋林[1] 奚秋磊[1] 蒋奕[1] 吴国豪[1] TAN Shan-jun;MENG Qing-yang;ZHUANG Qiu-lin;XI Qiu-lei;JIANG Yi;WU Guo-hao(Department of General Surgery/Shanghai Clinical Nutrition Research Center,Zhongshan Hospital,Fudan University,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院普通外科上海市临床营养研究中心,上海200032

出　　处：《肠外与肠内营养》2020年第5期261-264,共4页Parenteral & Enteral Nutrition

基　　金：国家自然科学基金青年科学基金项目(81900484);中国博士后科学基金资助项目(2019M661370);上海市青年科技英才扬帆计划(18YF1404700);上海市自然科学基金(19ZR1409100);上海市卫生健康系统重要薄弱学科建设计划-临床营养学(2019ZB0105);复旦大学附属中山医院青年科学基金(2016ZSQN56)。

摘　　要：目的:探讨美托洛尔对大鼠腹腔暴露后肠黏膜屏障的保护作用。方法:建立腹腔暴露大鼠模型,将24只大鼠随机分为三组:对照组、腹腔暴露组和美托洛尔组(腹腔暴露+美托洛尔干预,5 mg/kg),每组8只。观察各组大鼠小肠组织的病理变化,检测体外肠道通透性和血清D-乳酸变化,并通过培养肝、脾、肠系膜淋巴结细菌观察肠道细菌易位情况。结果:腹腔暴露组较对照组大鼠肠黏膜损伤Chiu’s评分,体外肠道通透性,血清D-乳酸和肠道细菌易位明显增加(P<0.05);美托洛尔组较腹腔暴露组大鼠肠黏膜损伤Chiu’s评分,体外肠道通透性,血清D-乳酸和肠道细菌易位明显降低(P<0.05)。结论:美托洛尔能降低腹腔暴露后的肠黏膜损伤,有助于保护腹部手术后的肠黏膜屏障功能。Objective:To investigate the protective effect of metoprolol on intestinal mucosal barrier after open abdomen in rats.Methods:A rat model of open abdomen was established.Twenty-four rats were randomized into three groups:control group,open abdomen group,and metoprolol group(open abdomen plus 5 mg/kg metoprolol intervention),respectively(n=8 each).The terminal ileum was sampled to observe histological changes and to analyze Chiu’s score for damage of intestinal mucosa.Blood was obtained to determinate serum D-lactate;terminal ileum was sampled to determinate in vitro intestinal permeability;liver,spleen and mesenteric lymph nodes were excised for evaluation of bacterial translocation.Results:Compared to control group,open abdomen group showed a significant increase in Chiu’s score for damage of intestinal mucosa,serum D-lactate,in vitro intestinal permeability,and bacterial translocation rate(P<0.05).Compared to open abdomen group,metoprolol group showed a significant decrease in Chiu’s score for damage of intestinal mucosa,serum D-lactate,in vitro intestinal permeability,and bacterial translocation rate(P<0.05).Conclusion:The data suggest that metoprolol could decrease intestinal mucosal damage induced by open abdomen,which is conducive to maintain intestinal mucosal barrier function after abdominal surgery.

关 键 词：美托洛尔 肠黏膜屏障 细菌易位 腹部手术 腹腔暴露 

分 类 号：R574[医药卫生—消化系统]