Isolation of Penicillium expansum WH-3 for the production of L(+)-tartaric acid  

L(+)-酒石酸生产菌株扩展青霉WH-3的筛选

作　　者：Wen-na BAO Yi CHEN Hong-xiu LIAO Hang CHEN Shi-wang LIU Yong LIU 

机构地区：[1]School of Biological and Chemical Engineering,Zhejiang University of Science and Technology,Hangzhou 310023,China [2]Zhejiang Provincial Key Laboratory for Chemical and Biological Processing Technology of Farm Products,Hangzhou 310023,China1

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2020年第10期835-840,共6页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Zhejiang Provincial Natural Science Foundation of China(No.LQ19C200001);he Zhejiang Xinmiao Talent Project(No.2020R415014),China。

摘　　要：The L(+)-form of tartaric acid(I(+)-TA)exists extensively in nature,and is widely used in the food,chemical,textile,building,and pharmaceutical in-dustries(Su et al.,2001).The main method for L(+)-TA production is microbial transformation by cis-epoxysuccinate hydrolase(CESH),which can catalyze the asymmetric hydrolysis of cis epoxysuccinic acid or its salts to TA or tartrate(Bao et al.,2019).Seventeen species containing CESH have been isolated so far.However,most species for L(+)-TA production have been reported from bacteria(Xuan and Feng,2019).The only fungus isolated from soil by our lab recently,that could be used as catalyst for the process under acidic condition,is Aspergillus niger WH-2(Bao et al,2020).In order to find strains with new characteristics,this study attempted to isolate a new CESH source from fungi and investigate its application value.L(+)-酒石酸生产菌株扩展青霉WH-3的筛选目的:筛选产L(+)-酒石酸的新真菌菌株。创新点:首次筛选获得能同时在酸性和高底物浓度下转化生产L(+)-酒石酸的菌株。方法:利用电镜、内转录间隔区(ITS)序列、核磁共振、质谱和旋光度对筛选菌株进行鉴定,并优化其发酵条件,研究其酶学性质。结论:扩展青霉WH-3的60%活性位于菌丝体中,且该菌丝体能在酸性(pH4.5)、高浓度底物中转化,具有高转化率和高立体特异性,为L(+)-酒石酸的生产提供新的催化剂。

关 键 词：L(+)-酒石酸:扩展青霉 顺式环氧琥珀酸水解酶:筛菌 

分 类 号：TQ921[轻工技术与工程—发酵工程]