Pannexin 1,a large-pore membrane channel,contributes to hypotonicity-induced ATP release in Schwann cells  

作　　者：Zhong-Ya Wei Hui-Lin Qu Yu-Juan Dai Qian Wang Zhuo-Min Ling Wen-Feng Su Ya-Yu Zhao Wei-Xing Shen Gang Chen 

机构地区：[1]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province,China [2]Medical School of Nantong University,Nantong,Jiangsu Province,China [3]Department of Anesthesiology,Affiliated Hospital of Nantong University,Nantong,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2021年第5期899-904,共6页中国神经再生研究（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China,Nos.31900718(to ZYW),31872773(to GC);the National Key Research and Development Program of China,No.2017YFA0104704(to GC);Basic Research Program of the Education Department of Jiangsu Province of China,Nos.19KJB180024(to ZYW),18KJB180020(to WXS);Postdoctoral Science Foundation of China,No.2019M651925(to ZYW),Jiangsu Students’Platform for Innovation and Entrepreneurship Training Program of China,No.201810304031Z(to YJD);Six Talent Peaks Project in Jiangsu Province of China,No.WSN-007(to WXS).

摘　　要：Pannexin 1(Panx 1),as a large-pore membrane channel,is highly permeable to ATP and other signaling molecules.Previous studies have demonstrated the expression of Panx 1 in the nervous system,including astrocytes,microglia,and neurons.However,the distribution and function of Panx 1 in the peripheral nervous system are not clear.Blocking the function of Panx 1 pharmacologically(carbenoxolone and probenecid)or with small interfering RNA targeting pannexins can greatly reduce hypotonicity-induced ATP release.Treatment of Schwann cells with a Ras homolog family member(Rho)GTPase inhibitor and small interfering RNA targeting Rho or cytoskeleton disrupting agents,such as nocodazole or cytochalasin D,revealed that hypotonicity-induced ATP release depended on intracellular RhoA and the cytoskeleton.These findings suggest that Panx 1 participates in ATP release in Schwann cells by regulating RhoA and the cytoskeleton arrangement.This study was approved by the Animal Ethics Committee of Nantong University,China(No.S20180806-002)on August 5,2018.

关 键 词：ATP CYTOSKELETON injury neuron pannexin 1 peripheral nerve Ras homolog family member A Schwann cells 

分 类 号：R446[医药卫生—诊断学] R741[医药卫生—临床医学]