基于IBR模型研究BDE-47和BDE-153对半滑舌鳎的毒性效应  

Toxic Effects of BDE-47 and BDE-153 on Cynoglossus semilaevis Gunther Based on IBR Model

作　　者：迟潇 陈碧鹃[2,3] 孙雪梅 朱琳[2,3] 唐学玺 夏斌[2,3] 曲克明 Chi Xiao;Chen Bijuan;Sun Xuemei;Zhu Lin;Tang Xuexi;Xia Bin;Qu Keming(College of Marine Life Sciences,Ocean University of China,Qingdao 266071,China;Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture,Key Laboratory of Fishery Resources and Ecological Environment in Shandong Province,Yellow Sea Fishery Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;Laboratory for Marine Ecology and Environmental Science,Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]中国海洋大学海洋生命学院,青岛266071 [2]中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续发展重点实验室,山东省渔业资源与生态环境重点实验室,青岛266071 [3]青岛海洋科学与技术试点国家实验室,海洋生态与环境科学功能实验室,青岛266237

出　　处：《生态毒理学报》2020年第4期192-202,共11页Asian Journal of Ecotoxicology

基　　金：中国水产科学研究院基本科研业务费资助项目(2017HY-ZD0202,2020TD12);国家自然科学基金委-山东省联合基金资助项目(U1606404)。

摘　　要：多溴联苯醚(PBDEs)是应用广泛的溴代阻燃剂。选择2,2’,4,4’-四溴联苯醚(BDE-47)和2,2’,4,4’,5,5’-六溴联苯醚(BDE-153)对半滑舌鳎(Cynoglossus semilaevis Gunther)进行15 d的暴露实验,测定了不同暴露浓度下半滑舌鳎肝脏的超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、7-乙氧基-3-异吩恶唑酮-脱乙基酶(7-ethoxyresorufin-o-deethylase,EROD)的活性、雌激素受体(estrogen receptor,ER)和丙二醛(malondialdehyde,MDA)含量,并运用综合生物标志物响应(integrated biomarker response,IBR)模型研究BDE-47和BDE-153对半滑舌鳎的毒性效应。结果表明,环境浓度BDE-47和BDE-153暴露下半滑舌鳎的抗氧化酶活性、ER含量和EROD活性无显著变化;中高浓度组(500 ng·L^-1和50000 ng·L^-1)与对照组基本上都有显著差异。运用IBR方法进行计算发现,BDE-47和BDE-153对半滑舌鳎的毒性效应呈现出显著的剂量效应。将2种污染物暴露组IBR值进行比较,发现BDE-47各浓度组的IBR值均大于BDE-153组,这表明BDE-47的毒性要高于BDE-153。IBR指数能够有效地对PBDEs的海洋环境风险进行科学评价。Polybrominated diphenyl ethers(PBDEs)are widely used as brominated flame retardants.In this study,Cynoglossus semilaevis Gunther was exposed to 2,2’,4,4’-tetrabrominated diphenyl ether(BDE-47)or 2,2’,4,4’,5,5’-hexabrominated diphenyl ether(BDE-153)alone for 15 d.Malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),estrogen receptor(ER)and 7-ethoxyresorufin-o-deethylase(EROD)of the liver in C.semilaevis under different exposure concentrations were measured.Integrated bio-marker response(IBR)model was used to elucidate the toxic effects of BDE-47 and BDE-153 on C.semilaevis.The results showed that BDE-47 and BDE-153 with environmental relevant concentration had no significant effects on the antioxidant enzyme activities,ER contents and EROD activity.While,significant differences in the medium and high concentration treatments(500 ng·L^-1 and 50000 ng·L^-1)were observed when compared with the control group.BDE-47 and BDE-153 showed significant dose-effects on C.semilaevis using IBR method.It was found that the IBR values of BDE-47 in each concentration treatment were higher than those of BDE-153,which indicated that the toxicity of BDE-47 was higher than BDE-153.Therefore,IBR model can effectively evaluate the risk of PBDEs in marine environments.

关 键 词：BDE-47 BDE-153 半滑舌鳎 毒性效应 综合生物标志物响应 

分 类 号：X171.5[环境科学与工程—环境科学]