大蒜素对冠心病大鼠心脏的保护作用及对主动脉形态、心肌细胞CaSR蛋白和凋亡指数的影响  

Protective effect of allicin on heart and its influence on aortic morphology,cardiomyocyte calcium sensing receptor and apoptotic index in rats with coronary heart disease

作　　者：许文克 史永恩 孟祥斌 张卫杰 高传玉 Xu Wenke;Shi Yong'en;Meng Xiangbin;Zhang Weijie;Gao Chuanyu(Emergency Department,Fuwai Central China Cardiovascular Hospital,Henan Province,Zhengzhou 450000,China;不详)

机构地区：[1]阜外华中心血管病医院急诊科,郑州450000

出　　处：《中国循证心血管医学杂志》2020年第10期1236-1239,1243,共5页Chinese Journal of Evidence-Based Cardiovascular Medicine

摘　　要：目的探究大蒜素对冠心病(CHD)大鼠心脏的保护作用及对主动脉形态、心肌细胞钙敏感受体(CaSR)和凋亡指数的影响。方法36只SD大鼠随机分为对照组、CHD组和CHD+大蒜素组(各为12只)。通过高脂饲料构建CHD模型,CHD+大蒜素组大鼠使用大蒜素灌胃20 mg/kg·d。通过ELISA分析大蒜素对CHD大鼠心肌酶指标和氧化应激指标的影响。HE染色分析主动脉损伤情况,比较各组心肌组织凋亡和CaSRmRNA和蛋白水平。结果3组大鼠各指标比较均有统计学意义(P<0.05)。CHD组的乳酸脱氢酶(LD)为(2201.56±289.04)IU/L和肌酸激酶同工酶(CK-MB)为(1286.92±136.69)IU/L均显著高于对照组(P<0.05),CHD+大蒜素组的LD(1828.59±264.36)IU/L和CK-MB(905.28±131.43)IU/L水平显著低于CHD组(P<0.05)。CHD组的内皮素-1(ET-1)为(1.80±0.21)pg/mL高于对照组,而一氧化氮(NO)浓度(32.69±6.70)μmol/L低于对照组(P<0.05)。CHD+大蒜素组的ET-1(1.54±0.178 pg/ml)显著低于CHD组,而NO(39.57±8.34μmol/L)显著高于CHD组(P<0.05)。CHD组的MDA(9.78±1.12 nmol/ml)高于对照组而SOD(116.04±11.67)U/ml低于对照组(P<0.05)。CHD+大蒜素组的MDA(5.62±0.95)nmol/ml显著低于CHD组,而SOD(147.58±12.18)显著高于CHD组(P<0.05)。CHD组的主动脉血管壁明显增厚,细胞膨大、排列紊乱。CHD+大蒜素组动脉壁增厚情况有所改善。CHD组凋亡指数(2.35±0.45)%显著高于对照组(P<0.05),CHD+大蒜素组凋亡指数(5.93±0.82)%显著低于CHD组(P<0.05)。CHD组的CaSRmRNA和蛋白水平均显著高于对照组(P<0.05),CHD+大蒜素组的CaSRmRNA和蛋白水平显著低于CHD组(P<0.05)。结论大蒜素可通过抑制CaSR的表达以及氧化应激反应保护血管内皮功能,保护心肌组织损伤,对CHD大鼠模型起到缓解作用。Objective To study the protective effect of allicin on heart and its influence on aortic morphology,cardiomyocyte calcium sensing receptor(CaSR)and apoptotic index in rats with coronary heart disease(CHD).Methods SD rats(n=36)were randomly divided into control group,CHD group and CHD+allicin group(each n=12).CHD model was established by high-fat diet,and CHD+allicin was intragastrically given allicin(20 mg/kg·d).The influence of allicin on myocardial enzyme indexes and oxidative stress indexes were analyzed by using ELISA in CHD rats.The level of myocardial apoptosis and mRNA and protein levels of CaSR were compared among all groups after HE staining.Results The comparison in all indexes had statistical significance among 3 groups(P<0.05).The levels of LD[(2201.56±289.04)IU/L]and CK-MB[(1286.92±136.69)IU/L]were significantly higher in CHD group than those in control group(P<0.05).The levels of LD[(1828.59±264.36)IU/L]and CK-MB[(905.28±131.43)IU/L]were significantly lower in CHD+allicin group than those in CHD group(P<0.05).The level of ET-1[(1.80±0.21)pg/ml]was higher and level of NO[(32.69±6.70)μmol/L]was lower in CHD group than those in control group(P<0.05).The level of ET-1[(1.54±0.178)pg/ml]was significantly lower,and level of NO[(39.57±8.34)μmol/L]was significantly higher in CHD+allicin group than those in CHD group(P<0.05).The level of MDA[(9.78±1.12)nmol/ml]was higher and level of SOD[(116.04±11.67)U/ml]was lower in CHD group than those in control group(P<0.05).The level of MDA[(5.62±0.95)nmol/ml]was significantly lower and level of SOD[(147.58±12.18)U/ml]was significantly higher in CHD+allicin group than those in CHD group(P<0.05).The aortic vessel wall was thicker significantly with enlarged cells and disorder arrangement in CHD group.The thickened aortic vessel wall was relieved in CHD+allicin group.The apoptosis index[(2.35±0.45)%]was significantly higher in CHD group than that in control group(P<0.05),and that[(5.93±0.82)%]was significantly lower in CHD+allicin group than that in CHD

关 键 词：冠心病 大蒜素 主动脉 氧化应激 凋亡 钙敏感受体 大鼠 

分 类 号：R541.4[医药卫生—心血管疾病]