神经节苷脂对慢性酒精中毒小鼠海马Caspase-3、Bax、Bcl-2蛋白表达水平的影响  

The effect of ganglioside on the expression of Caspase-3,Bax,Bcl-2 in the hippocampus of mice with chronic alcoholism

作　　者：康传依 赵娜[1] Mavlikhanova Asiya 胡建[1] Kang Chuanyi;Zhao Na;Mavlikhanova Asiya;Hu Jian(Department of Psychiatry,the First Affiliated Hospital of Harbin Medical University,Harbin 150001,China;Bashkir State Medical University,Bashkortostan 450008,Russia)

机构地区：[1]哈尔滨医科大学附属第一医院精神卫生中心,150001 [2]俄罗斯巴什基尔国立医科大学,450008

出　　处：《中华精神科杂志》2020年第5期426-430,共5页Chinese Journal of Psychiatry

基　　金：国家重点研发计划项目(2018YFC1314402)。

摘　　要：目的观察神经节苷脂(ganglioside,GM1)对慢性酒精中毒小鼠海马组织Caspase-3、Bax、Bcl-2蛋白表达水平的影响。方法将40只雄性Balb/c小鼠采用随机区组分组法分为慢性酒精中毒组、对照组、低剂量GM1治疗组、高剂量GM1治疗组,每组10只;慢性酒精中毒组采用酒精灌胃法建立慢性酒精中毒小鼠模型(酒精水溶液浓度逐渐从5%增加至35%),对照组使用等量生理盐水灌胃,低、高剂量治疗组在酒精灌胃后分别给予GM1(每天10、30 mg/kg)腹腔注射。于灌胃4周后进行酒精偏好实验测试及行为学测试,蛋白质印迹法测定4组小鼠海马组织Caspase-3、Bax和Bcl-2蛋白表达水平,组间比较采用单因素方差分析,两两比较采用Bonferroni法。结果4组酒水消耗率差异有统计学意义(F=630.83,P<0.05),对照组(18.9%±2.2%)和高剂量GMI治疗组(50.9%±3.2%)酒水消耗率低于慢性中毒组(56.5%±3.0%)。行为学测试结果显示4组悬尾试验累积不动时间(F=379.52)、避暗实验进入暗室潜伏期(F=7001.18)和进入暗室错误次数(F=33.87)以及疲劳转棒实验小鼠跌落时间(F=305.06)差异均有统计学意义(均P<0.05),且慢性酒精中毒组行为学测试结果均低于对照组,而高剂量GM1治疗组优于慢性酒精中毒组,差异均有统计学意义(P<0.05)。蛋白质印迹法示,4组Caspase-3、Bax、Bcl-2蛋白表达水平差异均有统计学意义(F=12.42、14.07、242.37,均P<0.05)。与慢性酒精中毒组相比,对照组和高剂量GM1治疗组海马组织Caspase-3蛋白和Bax蛋白表达水平显著降低(P<0.05),而Bcl-2蛋白在对照组、高剂量GM1治疗组、低剂量GM1治疗组海马区的表达水平显著高于慢性酒精中毒组(P<0.05)。结论GM1可以下调慢性酒精中毒小鼠海马组织Caspase-3、Bax蛋白表达水平,提高Bcl-2蛋白的表达水平。Objective To observe the effect of ganglioside(GM1)on the expression of Caspase-3,Bax and Bcl-2 in hippocampus of mice with chronic alcoholism.Methods Forty male Balb/c mice were randomly allocated into chronic alcoholism group(n=10),control group(n=10),low dose GM1 treatment group(n=10)and high dose GM1 treatment group(n=10).The mouse model of chronic alcoholism was established by intragastric administration of alcohol in the chronic alcoholism group(the concentration of alcohol solution gradually increased from 5%to 35%),mice in the control group were given the same amount of normal saline,while those in the low and high dose treatment groups were given intraperitoneal injection of GM1(10,30 mg/kg per day)after intragastric administration of alcohol.Four weeks after oral administration,alcohol preference test and behavior test were performed to determine the expression levels of Caspase-3,Bax and Bcl-2 in hippocampus.One-way ANOVA was used for comparison between groups,and Bonferroni method was used for pairwise comparison.Results There were significant differences of alcohol consumption rate among the four groups(F=630.83,P<0.05).The consumption rate of control group(18.9%±2.2%)and high dose GM1 treatment group(50.9%±3.2%)was lower than that of chronic alcoholism group(56.5%±3.0%).Similarly,there were also significant differences of behavior test result among the four groups(F=379.52,7001.18,33.87,305.06,all P<0.05).The results in chronic alcoholism group were lower than that of control group,and high dose GM1 treatment group were better than those in chronic alcoholism group(P<0.05).Results of Western Blot showed that there were significant differences in the expression of Caspase-3,Bax and Bcl-2 proteins among the four groups(F=12.42,14.07,242.37,all P<0.05).Compared to chronic alcoholism group,the expression of Caspase-3 protein and Bax protein in hippocampus of control group and high dose GM1 treatment group decreased significantly,while the expression level of Bcl-2 protein in hippocampus of control gr

关 键 词：酒精中毒 神经节苷脂类 印迹法 蛋白质 CASPASE-3 BAX BCL-2 

分 类 号：R749.62[医药卫生—神经病学与精神病学]