榔梅果实转录组分析及其柠檬酸生物合成途径关键酶基因结构与功能预测  

Transcriptome analysis of"Langmei"fruits and key enzyme genes structure and function prediction involved in citric acid biosynthesis

作　　者：欧金梅[1,2,3] 杨许 单春苗 张声祥 施圆圆[1] 吴家文 黄璐琦[2] 王瑞[1] OU Jin-mei;YANG Xu;SHAN Chun-miao;ZHANG Sheng-xiang;SHI Yuan-yuan;WU Jia-wen;HUANG Lu-qi;WANG Rui(Anhui University of Chinese Medicine,Hefei 230012,China;National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Key Laboratory of Xin'an Medicine,Ministry of Education,Anhui University of Chinese Medicine,Hefei 230038,China)

机构地区：[1]安徽中医药大学,安徽合肥230012 [2]中国中医科学院中药资源中心,北京100700 [3]安徽中医药大学新安医学教育部重点实验室,安徽合肥230038

出　　处：《中国中药杂志》2020年第19期4606-4616,共11页China Journal of Chinese Materia Medica

基　　金：安徽高校自然科学研究项目重点项目(KJ2019A0479);中央本级重大增减支项目子项目(2060302-1604-01)。

摘　　要：榔梅作为孑遗树种,果实极具药用价值。为研究榔梅柠檬酸的生物合成途径,采用Illumina HiSeq XTen高通量测序技术对榔梅果实进行转录组测序,经过拼接组装后得到38936条unigene,其中28311条unigene被公共数据库成功注释,15193条unigene映射到265条KEGG代谢通路;18908条unigene可归类到59个GO功能亚类。在柠檬酸合成与代谢途径中,共有103条unigene编码15个关键酶参与柠檬酸循环。对柠檬酸合酶进行序列分析和同源建模,结果显示榔梅的柠檬酸合酶是以α螺旋为主的同源二聚体蛋白,酶的活性中心氨基酸高度保守。该研究为榔梅果实中柠檬酸生物合成途径的解析奠定了基础,促进了榔梅基因资源的保护和开发。Prunus mume"Langmei"is a relict tree species,which fruit has good medicinal value.To understand the biosynthesis pathway of citric acid,the Illumina HiSeq XTen high-throughput sequencing technology was used to get the transcriptome from"Langmei".A total of 38936 unigenes were obtained by assembling the fruit transcripts,of which 28311 unigenes were successfully annotated in public databases,15193 unigenes were mapped to 265 KEGG metabolic pathways,and 18908 unigenes were classified into 59 GO functional subclasses,103 unigenes encoding 15 key enzymes involved in citric acid synthesis pathway were identified and analyzed.The structural model of citrate synthetase in"Langmei"showed that it was a homodimer and the secondary structure of each monomer was mainly composed of alpha helixes.Moreover,the residues in the active site of the citrate synthetase were highly conserved.This study provides a valuable resource for identifying candidate genes involved in the citric acid biosynthesis pathway,and will promote the development and sustainable utilization of genetic resources of"Langmei".

关 键 词：榔梅 转录组 基因注释 柠檬酸合成途径 柠檬酸合酶 

分 类 号：S567.19[农业科学—中草药栽培]